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Literature summary extracted from

  • Patel, R.N.; Hou, C.T.; Derelanko, P.
    Microbial oxidation of methanol: purification and properties of formaldehyde dehydrogenase from a Pichia sp. NRRL-Y-11328 (1983), Arch. Biochem. Biophys., 221, 135-142.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 1,10-phenanthroline 1 mM, 30% inhibition Komagataella pastoris
1.1.1.284 2,2'-dipyridyl 1 mM, 20% inhibition Komagataella pastoris
1.1.1.284 Cd2+ 1 mM, complete inhibition Komagataella pastoris
1.1.1.284 Cu2+
-
 Komagataella pastoris
1.1.1.284 Hg2+ 1 mM, complete inhibition Komagataella pastoris
1.1.1.284 imidazole 1 mM, 32% inhibition Komagataella pastoris
1.1.1.284 iodoacetamide 1 mM, 20% inhibition Komagataella pastoris
1.1.1.284 NEM 1 mM, 35% inhibition Komagataella pastoris
1.1.1.284 PCMB 1 mM, complete inhibition Komagataella pastoris
1.1.1.284 Zn2+ 1 mM, 40% inhibition Komagataella pastoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.12
-
 NAD+
-
 Komagataella pastoris
1.1.1.284 0.16
-
 reduced glutathione
-
 Komagataella pastoris
1.1.1.284 0.25
-
 NADH
-
 Komagataella pastoris
1.1.1.284 0.31
-
 formaldehyde
-
 Komagataella pastoris
1.1.1.284 0.6
-
 S-formylglutathione
-
 Komagataella pastoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 41000
-
 2 * 41000, SDS-PAGE Komagataella pastoris
1.1.1.284 84000
-
 gel filtration Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Komagataella pastoris
-
 NRRL-Y-11328
-
1.1.1.284 Komagataella pastoris NRRL-Y-11328
-
 NRRL-Y-11328
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 2090
-
-
 Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Komagataella pastoris S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Komagataella pastoris NRRL-Y-11328 S-formylglutathione + NADH + H+
-
 r
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Komagataella pastoris S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Komagataella pastoris NRRL-Y-11328 S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 S-formylglutathione + NADH
-
 Komagataella pastoris formaldehyde + glutathione + NAD+
-
 r
1.1.1.284 S-formylglutathione + NADH
-
 Komagataella pastoris NRRL-Y-11328 formaldehyde + glutathione + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 41000, SDS-PAGE Komagataella pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 8
-
 oxidation of formaldehyde Komagataella pastoris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.284 7 9 pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Komagataella pastoris
1.1.1.284 NADH
-
 Komagataella pastoris
1.1.1.284 NADP+ no activity with NADP+ Komagataella pastoris