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Literature summary extracted from

  • Hatanaka, T.; Asahi, N.; Tsuji, M.
    Purification and characterization of poly(vinyl alcohol) dehydrogenase from Pseudomonas sp. 113P3 (1995), Biosci. Biotechnol. Biochem., 59, 1813-1816.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.6 Ca2+ stimulates, the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added Pseudomonas sp.
1.1.2.6 Mg2+ stimulating effect is far less than with Ca2+, the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.6 67000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.6 Pseudomonas sp.
-
-
-
1.1.2.6 Pseudomonas sp. 113P3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.6
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.6 19.8
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.6 1,3-butanediol + oxidized 2,6-dichlorophenolindophenol 57% of activity with 2,4-pentanediol Pseudomonas sp. ?
-
 ?
1.1.2.6 1,3-butanediol + oxidized 2,6-dichlorophenolindophenol 57% of activity with 2,4-pentanediol Pseudomonas sp. 113P3 ?
-
 ?
1.1.2.6 1,3-cyclohexanediol + oxidized 2,6-dichlorophenolindophenol 393% of activity with 2,4-pentanediol Pseudomonas sp. ?
-
 ?
1.1.2.6 1,3-cyclohexanediol + oxidized 2,6-dichlorophenolindophenol 393% of activity with 2,4-pentanediol Pseudomonas sp. 113P3 ?
-
 ?
1.1.2.6 2,4-pentanediol + oxidized 2,6-dichlorophenolindophenol enzyme shows only activity against meso-2,4-pentanediol Pseudomonas sp. ?
-
 ?
1.1.2.6 2,4-pentanediol + oxidized 2,6-dichlorophenolindophenol enzyme shows only activity against meso-2,4-pentanediol Pseudomonas sp. 113P3 ?
-
 ?
1.1.2.6 additional information no activity with methanol, ethanol, 1-propanol, 1-butanol, 4-heptanol, 2-hexanol and 2-pentanol Pseudomonas sp. ?
-
 ?
1.1.2.6 additional information no activity with methanol, ethanol, 1-propanol, 1-butanol, 4-heptanol, 2-hexanol and 2-pentanol Pseudomonas sp. 113P3 ?
-
 ?
1.1.2.6 polyvinyl alcohol + oxidized 2,6-dichlorophenolindophenol activity is 6.7fold higher than activity with 2,4-pentanediol Pseudomonas sp. polyvinyl alcohol containing oxo-groups + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.6 polyvinyl alcohol + oxidized 2,6-dichlorophenolindophenol activity is 6.7fold higher than activity with 2,4-pentanediol Pseudomonas sp. 113P3 polyvinyl alcohol containing oxo-groups + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.6 monomer 1 * 67000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.2.6 PVA dehydrogenase
-
 Pseudomonas sp.
1.1.2.6 PVADH
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.6 37
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.6 7.2
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.6 heme hemequino-protein, 1.03 mol of heme per mol of apoenzyme Pseudomonas sp.
1.1.2.6 pyrroloquinoline quinone the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added Pseudomonas sp.