EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.0023 | - |
5alpha-Cholestan-3beta-ol | - |
Nocardia erythropolis | |
1.1.3.6 | 0.0029 | - |
cholesterol | - |
Nocardia erythropolis | |
1.1.3.6 | 0.0059 | - |
stigmasterol | - |
Nocardia erythropolis | |
1.1.3.6 | 0.0066 | - |
sitosterol | - |
Nocardia erythropolis | |
1.1.3.6 | 0.0115 | - |
fucosterol | - |
Nocardia erythropolis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | Nocardia erythropolis | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | Streptomyces violascens | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | Brevibacterium sterolicum | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | Brevibacterium sterolicum | - |
- |
- |
1.1.3.6 | Nocardia erythropolis | - |
- |
- |
1.1.3.6 | Streptomyces violascens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.6 | overview: purification procedures | Nocardia erythropolis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia erythropolis | |
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces violascens | |
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | 5alpha-cholestan-3beta-ol + O2 | - |
Streptomyces violascens | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | 5alpha-cholestan-3beta-ol + O2 | - |
Brevibacterium sterolicum | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | 5alpha-cholestan-3beta-ol + O2 | 87% of activity | Nocardia erythropolis | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | 7E,22-ergostadien-3beta-ol + O2 | - |
Nocardia erythropolis | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
1.1.3.6 | 7E,22-ergostadien-3beta-ol + O2 | - |
Streptomyces violascens | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
1.1.3.6 | 7E,22-ergostadien-3beta-ol + O2 | - |
Brevibacterium sterolicum | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
1.1.3.6 | beta-sitosterol + O2 | - |
Nocardia erythropolis | stigmast-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | beta-sitosterol + O2 | - |
Streptomyces violascens | stigmast-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | beta-sitosterol + O2 | - |
Brevibacterium sterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | campesterol + O2 | - |
Streptomyces violascens | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | campesterol + O2 | - |
Brevibacterium sterolicum | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | campesterol + O2 | 67% of activity with cholesterol | Nocardia erythropolis | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | overview: substrate specificity | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | overview: substrate specificity | Streptomyces violascens | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | overview: substrate specificity | Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | first step in cholesterol degradation | Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | first step in cholesterol degradation | Streptomyces violascens | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | first step in cholesterol degradation | Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | desmosterol + O2 | - |
Streptomyces violascens | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | desmosterol + O2 | - |
Brevibacterium sterolicum | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | desmosterol + O2 | 98% of activity with cholesteol | Nocardia erythropolis | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | ergosterol + O2 | - |
Nocardia erythropolis | dehydroergosterol + H2O2 | - |
? | |
1.1.3.6 | ergosterol + O2 | - |
Streptomyces violascens | dehydroergosterol + H2O2 | - |
? | |
1.1.3.6 | ergosterol + O2 | - |
Brevibacterium sterolicum | dehydroergosterol + H2O2 | - |
? | |
1.1.3.6 | fucosterol + O2 | - |
Nocardia erythropolis | stigmasta-5,24(28)-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | stigmasterol + O2 | - |
Nocardia erythropolis | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | stigmasterol + O2 | - |
Streptomyces violascens | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | stigmasterol + O2 | - |
Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? |