EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.013 | - |
cholesterol | Km increases when enzyme is entrapped in reverse micelles of the synthetic surfactant aerosol-OT-isooctane | Nocardia erythropolis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | Nocardia erythropolis | - |
cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | Nocardia erythropolis | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia erythropolis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.6 | commercial preparation | - |
Nocardia erythropolis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | - |
Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 40 | - |
enzyme after micellization | Nocardia erythropolis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 7.5 | - |
enzyme after micellization | Nocardia erythropolis |