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Literature summary extracted from

  • Kagawa, T.; Bruno, P.L.
    NADP-malate dehydrogenase from leaves of Zea mays: purification and physical, chemical, and kinetic properties (1988), Arch. Biochem. Biophys., 260, 674-695.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.82 dithiothreitol activates Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.82 0.024
-
 NADPH
-
 Zea mays
1.1.1.82 0.056
-
 oxaloacetate cosubstrate NADPH Zea mays
1.1.1.82 0.061
-
 oxaloacetate cosubstrate NADH Zea mays
1.1.1.82 0.073
-
 NADP+ cosubstrate malate Zea mays
1.1.1.82 0.8
-
 NAD+ cosubstrate malate Zea mays
1.1.1.82 0.83
-
 NADH cosubstrate oxaloacetate Zea mays
1.1.1.82 29
-
 (S)-malate cosubstrate NAD+ Zea mays
1.1.1.82 32
-
 (S)-malate cosubstrate NADP+ Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.82 43000
-
 2 * 43000, SDS-PAGE Zea mays
1.1.1.82 88000
-
 gradient pore-PAGE Zea mays
1.1.1.82 91000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.82 oxaloacetate + NADPH Zea mays key enzyme of the photosynthetic pathway of C4 plants (S)-malate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.82
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.82 leaf
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.82 945
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 (S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.82 (S)-malate + NADP+
-
 Zea mays oxaloacetate + NADPH + H+
-
 r
1.1.1.82 oxaloacetate + NADH
-
 Zea mays (S)-malate + NAD+
-
 r
1.1.1.82 oxaloacetate + NADPH
-
 Zea mays (S)-malate + NADP+
-
 r
1.1.1.82 oxaloacetate + NADPH key enzyme of the photosynthetic pathway of C4 plants Zea mays (S)-malate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.82 dimer 2 * 43000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.82 8.5
-
 NADPH-dependent reduction of oxaloacetate Zea mays