EC Number | General Stability | Organism |
---|---|---|
1.1.1.81 | activity is slowly lost on storage at -15°C interspersed with frequent thawing and re-freezing, about 20% activity is lost over 8 cycles of freezing and thawing | Paracoccus denitrificans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.81 | bromide | 33 mM, 38% inhibition | Paracoccus denitrificans | |
1.1.1.81 | citrate | competitive vs. hydroxypyruvate | Paracoccus denitrificans | |
1.1.1.81 | Iodide | 33 mM, 39% inhibition | Paracoccus denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.81 | hydroxypyruvate + NAD(P)H | Paracoccus denitrificans | - |
D-glycerate + NAD(P)+ + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.81 | Paracoccus denitrificans | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.81 | 0.077 | - |
oxidation of glycerate | Paracoccus denitrificans |
1.1.1.81 | 0.135 | - |
cofactor NADH | Paracoccus denitrificans |
1.1.1.81 | 0.33 | - |
cofactor NADPH | Paracoccus denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.81 | 2,3-butandione + NAD(P)H | 33 mM, 12% of activity with hydroxypyruvate | Paracoccus denitrificans | butan-2-ol-3-one + NAD(P)+ | - |
? | |
1.1.1.81 | acetoin + NAD(P)H + H+ | 33 mM, 14% of activity with hydroxypyruvate | Paracoccus denitrificans | 2,3-butanediol + NAD(P)+ | - |
? | |
1.1.1.81 | glyoxylate + NAD(P)H | 33 mM, 15% activity | Paracoccus denitrificans | glycolate + NAD(P)+ | - |
? | |
1.1.1.81 | hydroxypyruvate + NAD(P)H | - |
Paracoccus denitrificans | D-glycerate + NAD(P)+ + H+ | - |
? | |
1.1.1.81 | hydroxypyruvate + NAD(P)H | - |
Paracoccus denitrificans | D-glycerate + NAD(P)+ + H+ | - |
r | |
1.1.1.81 | oxaloacetate + NAD(P)H | 33 mM, 32% of activity with hydroxypyruvate | Paracoccus denitrificans | malate + NAD(P)+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.81 | 42 | - |
half-life of 35 min in 50 mM phosphate buffer, pH 7.0 | Paracoccus denitrificans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.81 | 5 | - |
sharp peak | Paracoccus denitrificans |