EC Number | General Stability | Organism |
---|---|---|
1.1.1.239 | stable at 4°C for at least 10-12 days in the presence of 10 mM 2-mercaptoethanol, activity increases 2fold in the first 2-3 days | Cavia porcellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.239 | Triton X-100 | immediate loss of 60-70% activity, remaining activity is stable for 4 days, competitive vs. testosterone, non-competitive vs. NAD+ | Cavia porcellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.239 | 0.0022 | - |
testosterone | membrane bound | Cavia porcellus | |
1.1.1.239 | 0.0073 | - |
testosterone | solubilized with Triton X-100 | Cavia porcellus | |
1.1.1.239 | 0.1 | - |
NAD+ | solubilized with Triton X-100 | Cavia porcellus | |
1.1.1.239 | 0.164 | - |
NAD+ | membrane bound | Cavia porcellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.239 | microsome | - |
Cavia porcellus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.239 | 176000 | - |
agarose gel chromatography and density gradient centrifugation of Triton X-100 solubilized microsomes | Cavia porcellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.239 | testosterone + NAD+ | Cavia porcellus | - |
androst-4-ene-3,17-dione + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.239 | Cavia porcellus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.239 | liver | - |
Cavia porcellus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.239 | testosterone + NAD+ | - |
Cavia porcellus | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.239 | 9.5 | - |
solubilized and membrane bound enzyme | Cavia porcellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.239 | 6 | 9.5 | sharp decrease above pH 10.0 | Cavia porcellus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.239 | NAD+ | - |
Cavia porcellus |