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Literature summary extracted from

  • Bailey-Serres, J.; Nguyen, M.T.
    Purification and characterization of cytosolic 6-phosphogluconate dehydrogenase in isozymes from maize (1992), Plant Physiol., 100, 1580-1583.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.43 NADPH
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.43 0.023
-
 NADP+ 7.5 mM MgCl2 Zea mays
1.1.1.43 0.0422
-
 NADP+
-
 Zea mays
1.1.1.43 0.14
-
 6-phospho-D-gluconate 7.5 mM MgCl2 Zea mays
1.1.1.43 0.15
-
 6-phospho-D-gluconate 1 mM MnCl2 Zea mays
1.1.1.43 0.25
-
 NADP+ 0.01 mM NADPH Zea mays
1.1.1.43 0.32
-
 6-phospho-D-gluconate
-
 Zea mays
1.1.1.43 0.39
-
 6-phospho-D-gluconate 0.01 mM NADPH Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.43 cytoplasm
-
 Zea mays 5737
-
1.1.1.43 cytosol
-
 Zea mays 5829
-
1.1.1.43 cytosol
-
 Ricinus communis 5829
-
1.1.1.43 plastid
-
 Zea mays 9536
-
1.1.1.43 proplastid
-
 Ricinus communis 9537
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.43 Mg2+
-
 [Candida] boidinii
1.1.1.43 Mg2+ stimulated by MgCl2 Ricinus communis
1.1.1.43 Mg2+ activity increases slightly with 7.5 mM MgCl2 Zea mays
1.1.1.43 Mn2+ activity increases slightly with 7.5 mM MnCl2 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.43 55000
-
 SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Spinacia oleracea
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Nicotiana tabacum
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ [Candida] boidinii
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Ricinus communis
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Zea mays enzyme belongs to the oxidative pentose phosphate pathway 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.43 Nicotiana tabacum
-
 tobacco
-
1.1.1.43 Ricinus communis
-
 castor bean
-
1.1.1.43 Spinacia oleracea
-
 spinach
-
1.1.1.43 Zea mays
-
 maize, L. Pioneer HiBred International line B73
-
1.1.1.43 [Candida] boidinii
-
 methanol utilizing yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.43
-
 Zea mays
1.1.1.43
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.43 endosperm
-
 Ricinus communis
-
1.1.1.43 leaf
-
 Spinacia oleracea
-
1.1.1.43 leaf
-
 Zea mays
-
1.1.1.43 root from seedlings Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.43 20.8
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Spinacia oleracea 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Nicotiana tabacum 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 [Candida] boidinii 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Ricinus communis 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ enzyme belongs to the oxidative pentose phosphate pathway Zea mays 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.43 dimer
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.43 7.8 8
-
 Ricinus communis