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Literature summary extracted from

  • Voegele, R.T.; Mitsch, M.J.; Finan, T.M.
    Characterization of two members of a novel malic enzyme class (1999), Biochim. Biophys. Acta, 1432, 275-285.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 ADP slight stimulstion Sinorhizobium meliloti
1.1.1.39 aspartate stimulates Sinorhizobium meliloti
1.1.1.39 ATP slight stimulstion Sinorhizobium meliloti
1.1.1.39 fructose 1,6-bisphosphate stimulates Sinorhizobium meliloti
1.1.1.39 fumarate stimulates Sinorhizobium meliloti
1.1.1.39 succinate stimulates Sinorhizobium meliloti

General Stability

EC Number General Stability Organism
1.1.1.40 loss of activity upon repeated freezing and thawing Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 acetyl-CoA potent inhibitor Sinorhizobium meliloti
1.1.1.39 bicarbonate
-
 Sinorhizobium meliloti
1.1.1.39 D-Tartrate
-
 Sinorhizobium meliloti
1.1.1.39 DL-lactate
-
 Sinorhizobium meliloti
1.1.1.39 glyoxylate
-
 Sinorhizobium meliloti
1.1.1.39 malonate
-
 Sinorhizobium meliloti
1.1.1.39 NADH
-
 Sinorhizobium meliloti
1.1.1.39 oxaloacetate
-
 Sinorhizobium meliloti
1.1.1.39 phosphoenolpyruvate
-
 Sinorhizobium meliloti
1.1.1.39 pyruvate
-
 Sinorhizobium meliloti
1.1.1.39 Tartronate
-
 Sinorhizobium meliloti
1.1.1.40 D-malate
-
 Sinorhizobium meliloti
1.1.1.40 D-Tartrate
-
 Sinorhizobium meliloti
1.1.1.40 glyoxylate
-
 Sinorhizobium meliloti
1.1.1.40 L-Tartrate
-
 Sinorhizobium meliloti
1.1.1.40 malonate
-
 Sinorhizobium meliloti
1.1.1.40 Tartronate
-
 Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.089
-
 NAD+
-
 Sinorhizobium meliloti
1.1.1.39 1.56
-
 NADP+
-
 Sinorhizobium meliloti
1.1.1.40 2.6
-
 L-malate
-
 Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 82000
-
 x * 82000, SDS-PAGE Sinorhizobium meliloti
1.1.1.40 82000
-
 x * 82000, SDS-PAGE Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Sinorhizobium meliloti
-
-
-
1.1.1.40 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Sinorhizobium meliloti
1.1.1.40
-
 Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 45.2
-
-
 Sinorhizobium meliloti
1.1.1.40 49
-
-
 Sinorhizobium meliloti

Storage Stability

EC Number Storage Stability Organism
1.1.1.39 -20°C, stable for several weeks Sinorhizobium meliloti
1.1.1.39 4°C, loss of activity upon prolonged storage Sinorhizobium meliloti
1.1.1.40 -20°C, stable for several weeks Sinorhizobium meliloti
1.1.1.40 4°C, loss of activity upon prolonged storage Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADPH
-
 r
1.1.1.39 malate + NAD+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADH + H+
-
 ir
1.1.1.39 additional information no decarboxylation of oxaloacetate Sinorhizobium meliloti ?
-
 ?
1.1.1.40 (S)-malate + NADP+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADPH
-
 ir
1.1.1.40 Oxaloacetate no activity Sinorhizobium meliloti Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.39 ? x * 82000, SDS-PAGE Sinorhizobium meliloti
1.1.1.40 ? x * 82000, SDS-PAGE Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 582
-
 L-malate assuming an octameric oligomerization state Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Sinorhizobium meliloti
1.1.1.39 NADP+ cofactor Sinorhizobium meliloti
1.1.1.40 NADP+
-
 Sinorhizobium meliloti