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Literature summary extracted from

  • Chistoserdova, L.V.; Lidstrom, M.E.
    Purification and characterization of hydroxypyruvate reductase from the facultative methylotroph Methylobacterium extorquens AM1 (1991), J. Bacteriol., 173, 7228-7232.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.29 Hydroxypyruvate product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM Methylorubrum extorquens
1.1.1.29 NADH concentration above 0.5 mM Methylorubrum extorquens
1.1.1.29 NADPH concentration above 0.5 mM Methylorubrum extorquens
1.1.1.81 citrate 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 25% of NADPH linked reduction Methylorubrum extorquens AM1
1.1.1.81 D-glycerate 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction Methylorubrum extorquens AM1
1.1.1.81 glycolate 5 mM, 15% of NADPH linked reduction Methylorubrum extorquens AM1
1.1.1.81 glyoxylate 10 mM, 55% inhibition of NADH linked hydroxypyruvate reduction, 15% of NADPH linked reduction, 80% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
1.1.1.81 Hydroxypyruvate 0.5 mM, 90% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
1.1.1.81 NAD+ 4 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 60% of NADPH linked reduction Methylorubrum extorquens AM1
1.1.1.81 NADP+ 4 mM, 15% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction, 8% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
1.1.1.81 oxaloacetate 2.5 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 20% of NADPH linked reduction Methylorubrum extorquens AM1
1.1.1.81 pyruvate 10 mM, 40% inhibition of NADH linked hydroxypyruvate reduction Methylorubrum extorquens AM1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.29 0.04
-
 NADH
-
 Methylorubrum extorquens
1.1.1.29 0.06
-
 NADPH
-
 Methylorubrum extorquens
1.1.1.29 0.1
-
 Hydroxypyruvate
-
 Methylorubrum extorquens
1.1.1.29 0.12
-
 Hydroxypyruvate
-
 Methylorubrum extorquens
1.1.1.29 1.5
-
 glyoxylate
-
 Methylorubrum extorquens
1.1.1.29 2.6
-
 glycerate
-
 Methylorubrum extorquens
1.1.1.29 20
-
 glyoxylate
-
 Methylorubrum extorquens
1.1.1.81 0.04
-
 NADH
-
 Methylorubrum extorquens AM1
1.1.1.81 0.06
-
 NADPH
-
 Methylorubrum extorquens AM1
1.1.1.81 0.1
-
 Hydroxypyruvate cofactor NADH Methylorubrum extorquens AM1
1.1.1.81 1.25
-
 Hydroxypyruvate cofactor NADPH Methylorubrum extorquens AM1
1.1.1.81 2.6
-
 glycerate
-
 Methylorubrum extorquens AM1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.29 37000
-
 2 * 37000, SDS-PAGE Methylorubrum extorquens
1.1.1.29 71000
-
 SDS-PAGE Methylorubrum extorquens
1.1.1.81 37000
-
 2 * 37000, SDS-PAGE Methylorubrum extorquens AM1
1.1.1.81 71000
-
 gradient PAGE Methylorubrum extorquens AM1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.81 hydroxypyruvate + NAD(P)H Methylorubrum extorquens AM1 cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH D-glycerate + NAD(P)+ + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.29 Methylorubrum extorquens
-
-
-
1.1.1.81 Methylorubrum extorquens AM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.29
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.81 675
-
-
 Methylorubrum extorquens AM1

Storage Stability

EC Number Storage Stability Organism
1.1.1.29 4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity Methylorubrum extorquens
1.1.1.81 4°C, 50 mM Tris-HCl, pH 7.5, 10% glycerol, 1 mM dithiothreitol, 2 months, 10% loss of activity Methylorubrum extorquens AM1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.29 D-glycerate + NAD+
-
 Methylorubrum extorquens hydroxypyruvate + NADH + H+
-
 r
1.1.1.29 glyoxylate + NADH
-
 Methylorubrum extorquens glycolate + NAD+
-
 ir
1.1.1.29 hydroxypyruvate + NADH
-
 Methylorubrum extorquens D-glycerate + NAD+
-
 r
1.1.1.29 hydroxypyruvate + NADPH + H+
-
 Methylorubrum extorquens D-glycerate + NADP+
-
 r
1.1.1.81 D-glycerate + NAD+
-
 Methylorubrum extorquens AM1 3-hydroxypyruvate + NADH + H+
-
 r
1.1.1.81 glyoxylate + NAD(P)H cofactor NADH, 15% of activity with hydroxypyruvate, cofactor NADPH, 1.5% of activity with hydroxypyruvate Methylorubrum extorquens AM1 glycolate + NAD(P)+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Methylorubrum extorquens AM1 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH Methylorubrum extorquens AM1 D-glycerate + NAD(P)+ + H+
-
 r
1.1.1.81 hydroxypyruvate + NADH + H+
-
 Methylorubrum extorquens AM1 D-glycerate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.29 dimer 2 * 37000, SDS-PAGE Methylorubrum extorquens
1.1.1.81 dimer 2 * 37000, SDS-PAGE Methylorubrum extorquens AM1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.29 4 6.5 reduction of hydroxypyruvate Methylorubrum extorquens
1.1.1.29 9 11 glycerate oxidation Methylorubrum extorquens
1.1.1.81 4.5
-
 broad optimum between pH 4.0 and 6.5 for hydroxypyruvate reduction Methylorubrum extorquens AM1
1.1.1.81 9 11 glycerate oxidation Methylorubrum extorquens AM1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.29 NADH
-
 Methylorubrum extorquens
1.1.1.29 NADPH
-
 Methylorubrum extorquens