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Literature summary extracted from

  • Akao, T.; Akao, T.; Hattori, M.; Namba, T.; Kobashi, K.
    Purification and properties of 3alpha-hydroxyglycyrrhetinate dehydrogenase of Clostridium innocuum from human intestine (1988), J. Biochem., 103, 504-507.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.230 0.00027
-
 NADP+
-
 [Clostridium] innocuum
1.1.1.230 0.001
-
 3-Ketoglycyrrhetic acid
-
 [Clostridium] innocuum
1.1.1.230 0.002
-
 3alpha-Hydroxyglycyrrhetic acid
-
 [Clostridium] innocuum
1.1.1.230 0.024
-
 NADPH
-
 [Clostridium] innocuum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.230 soluble
-
 [Clostridium] innocuum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.230 30000
-
 2 * 30000, SDS-PAGE [Clostridium] innocuum
1.1.1.230 53000
-
 gel filtration [Clostridium] innocuum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.230 3alpha-hydroxyglycyrrhetic acid + NADP+ [Clostridium] innocuum oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group 3-ketoglycyrrhetic acid + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.230 [Clostridium] innocuum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.230
-
 [Clostridium] innocuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.230 3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
-
 [Clostridium] innocuum 3-keto-18alpha-glycyrrhetic acid + NADPH
-
 r
1.1.1.230 3alpha-hydroxyglycyrrhetic acid + NADP+ oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group, not accepted as substrates: 3beta-hydroxyglycyrrhetic acid, steroids or bile acids containing 3alpha-hydroxy or 3-keto group [Clostridium] innocuum 3-ketoglycyrrhetic acid + NADPH
-
 r
1.1.1.230 3alpha-hydroxyglycyrrhetic acid + NADP+ oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group [Clostridium] innocuum 3-ketoglycyrrhetic acid + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.230 dimer 2 * 30000, SDS-PAGE [Clostridium] innocuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.230 6
-
 reduction of 3-keto group [Clostridium] innocuum
1.1.1.230 8
-
 oxidation of 3alpha-hydroxy group [Clostridium] innocuum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.230 NADP+
-
 [Clostridium] innocuum
1.1.1.230 NADPH
-
 [Clostridium] innocuum