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Literature summary extracted from

  • Campbell, R.E.; Sala, R.F.; van de Rijn, I.; Tanner, M.E.
    Properties and kinetic analysis of UDP-glucose dehydrogenase from group A streptococci (1997), J. Biol. Chem., 272, 3416-3422.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.22 pharmacology target for inhibitor design Streptococcus pyogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.22 overexpression in Escherichia coli JM109 Streptococcus pyogenes

General Stability

EC Number General Stability Organism
1.1.1.22 fairly stable with 2 mM dithiothreitol Streptococcus pyogenes
1.1.1.22 stable during repeated freezing and thawing cycles with 10% glycerol Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.22 thiol group modifying reagents
-
 Streptococcus pyogenes
1.1.1.22 UDP-D-xylose competitive with UDPglucose Streptococcus pyogenes
1.1.1.22 Uridine 5'-diphosphate chloroacetol alkylates a thiol group of cysteine in the catalytic centre via being specifically bound instead of the substrate Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.22 0.02
-
 UDPglucose
-
 Streptococcus pyogenes
1.1.1.22 0.06
-
 NAD+
-
 Streptococcus pyogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.22 45500
-
 electrospray mass spectrometry Streptococcus pyogenes
1.1.1.22 52000
-
 gel filtation Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.22 UDPglucose + NAD+ + H2O Streptococcus pyogenes delivers glucuronic acid for the formation of a antiphagocytic polysaccharide capsule that is required for virulence of pathogenic bacteria UDPglucuronate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Streptococcus pyogenes
-
 group A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22 recombinant Streptococcus pyogenes

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.22 UDP-alpha-D-glucose + 2 NAD+ + H2O = UDP-alpha-D-glucuronate + 2 NADH + 2 H+ ping-pong reaction mechanism Streptococcus pyogenes
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.22 1.5
-
 recombinant and purified Streptococcus pyogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.22 5°C, 50 mM Tris-HCl, pH 8.7, 2 mM dithiothreitol, after 24 h 90% activity left Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 UDP-glucose + 2 NAD+ + H2O
-
 Streptococcus pyogenes UDP-glucuronate + 2 NADH + 2 H+
-
 ?
1.1.1.22 UDPglucose + NAD+ + H2O delivers glucuronic acid for the formation of a antiphagocytic polysaccharide capsule that is required for virulence of pathogenic bacteria Streptococcus pyogenes UDPglucuronate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.22 monomer 1 * 45500, SDS-PAGE Streptococcus pyogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.22 30
-
 after incubation for 1.6 h loss of 40% activity Streptococcus pyogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.22 0.117
-
 UDPglucose
-
 Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.22 additional information no constantly bound chromophoric cofactors, i.e. NAD+, required Streptococcus pyogenes