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Literature summary extracted from

  • Yamano, S; Kageura, E.; Ishida, T.; Toki, S.
    Purification and characterization of guinea pig liver morphine 6-dehydrogenase (1985), J. Biol. Chem., 260, 5259-5264.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.218 2-mercaptoethanol reversible Cavia porcellus

General Stability

EC Number General Stability Organism
1.1.1.218 2-mercaptoethanol stabilizes during purification and storage, removal inactivates, reversible Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.218 CdCl2 dithiol modifier, strong competitive inhibitor to cofactor Cavia porcellus
1.1.1.218 additional information no inhibition by NEM, PCMB, iodoacetic acid Cavia porcellus
1.1.1.218 Phenylarsine oxide dithiol modifier, competitive inhibitor to cofactor binding and noncompetitively toward morphine binding Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.218 0.015
-
 nalorphine cosubstrate NAD+ Cavia porcellus
1.1.1.218 0.033
-
 codeine with NADP+ Cavia porcellus
1.1.1.218 0.044
-
 normorphine cosubstrate NAD+ Cavia porcellus
1.1.1.218 0.048
-
 nalorphine cosubstrate NADP+ Cavia porcellus
1.1.1.218 0.079
-
 codeine with NAD+ Cavia porcellus
1.1.1.218 0.12
-
 morphine cosubstrate NAD+ Cavia porcellus
1.1.1.218 0.15
-
 normorphine cosubstrate NADP+ Cavia porcellus
1.1.1.218 0.4
-
 ethylmorphine cosubstrate NAD+ Cavia porcellus
1.1.1.218 0.49
-
 morphine cosubstrate NADP+ Cavia porcellus
1.1.1.218 0.54
-
 Dihydrocodeine cosubstrate NAD+ Cavia porcellus
1.1.1.218 1.54
-
 ethylmorphine cosubstrate NADP+ Cavia porcellus
1.1.1.218 2.11
-
 Dihydrocodeine cosubstrate NADP+ Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.218 cytosol
-
 Cavia porcellus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.218 29000
-
 gel filtration Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.218 Cavia porcellus
-
-
-
1.1.1.218 Cavia porcellus Hartley
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.218 gel filtration, various chromatographic techniques Cavia porcellus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.218 morphine + NAD(P)+ = morphinone + NAD(P)H + H+ dehydrogenation of the 6-hydroxy group of morphine derivatives having a 7,8-unsaturated bond Cavia porcellus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.218 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.218 0.53
-
 morphine, NAD+ Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
1.1.1.218 2-4°C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.218 codeine + NAD(P)+ oxidation at 80% the rate of morphine oxidation Cavia porcellus codeinone + NAD(P)H
-
 ?
1.1.1.218 codeine + NAD(P)+ oxidation at 80% the rate of morphine oxidation Cavia porcellus Hartley codeinone + NAD(P)H
-
 ?
1.1.1.218 dihydrocodeine + NADP+
-
 Cavia porcellus dihydrocodeinone + NADPH
-
 ?
1.1.1.218 dihydrocodeine + NADP+
-
 Cavia porcellus Hartley dihydrocodeinone + NADPH
-
 ?
1.1.1.218 ethylmorphine + NAD(P)+ oxidation at 51% the rate of morphine oxidation Cavia porcellus ?
-
 ?
1.1.1.218 morphine + NADP+
-
 Cavia porcellus morphinone + NADPH + H+ production of morphinone demonstrated in vitro experiments ?
1.1.1.218 morphine + NADP+
-
 Cavia porcellus Hartley morphinone + NADPH + H+ production of morphinone demonstrated in vitro experiments ?
1.1.1.218 nalorphine + NAD(P)+ best substrate, oxidation at 120% the rate of morphine oxidation Cavia porcellus (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+
-
 ?
1.1.1.218 nalorphine + NAD(P)+ best substrate, oxidation at 120% the rate of morphine oxidation Cavia porcellus Hartley (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+
-
 ?
1.1.1.218 normorphine + NAD(P)+ about 3 times better substrate than morphine Cavia porcellus ?
-
 ?
1.1.1.218 normorphine + NAD(P)+ about 3 times better substrate than morphine Cavia porcellus Hartley ?
-
 ?
1.1.1.218 trans-1,4-decalindiol + NADP+ about 30% of the activity observed with morphine Cavia porcellus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.218 25
-
 enzyme assay Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.218 9.1
-
 with NADP+ as cofactor, in tricine-NaOH buffer Cavia porcellus
1.1.1.218 9.7
-
 with NAD+ as cofactor, in glycine-NaCl-NaOH buffer Cavia porcellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.218 6
-
 in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NADP+ or NAD+ at least for a week stable at 2-4°C Cavia porcellus
1.1.1.218 8
-
 in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NAD+ at least for a week stable at 2-4°C Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.218 NAD(P)+ utilized both with nearly equal efficiency Cavia porcellus