BRENDA - Enzyme Database

Identification of two dihydrodiol dehydrogenases associated with 3(17)alpha-hydoxysteroid dehydrogenase activity in mouse kidney

Nakagawa, M.; Tsukuda, F.; Nakayama, T.; Matsuura, K.; Hara, A.; Sawada, H.; J. Biochem. 106, 633-638 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.209
17beta-estradiol
5 mM, 77% inhibition
Mus musculus
1.1.1.209
5alpha-Androstan-3beta-ol-17-one
1 mM, 80% inhibition
Mus musculus
1.1.1.209
5alpha-pregnan-3beta-ol-21-one
1 mM, 66% inhibition
Mus musculus
1.1.1.209
5beta-androstan-3beta-ol-17-one
1 mM, 91% inhibition
Mus musculus
1.1.1.209
5beta-Pregnan-3beta-ol-20-one
1 mM, 80% inhibition
Mus musculus
1.1.1.209
Barbital
100 mM, 11% inhibition
Mus musculus
1.1.1.209
Dienstrol
1 mM, 22% inhibition
Mus musculus
1.1.1.209
Hexestrol
1 mM, 77% inhibition
Mus musculus
1.1.1.209
Stilbestrol
10 mM, 75% inhibition
Mus musculus
1.1.1.209
testosterone
1 mM, 48% inhibition
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.209
0.0001
-
Epitestosterone
cofactor NADP+
Mus musculus
1.1.1.209
0.0001
-
5beta-androstane-3alpha,17beta-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0003
-
5beta-androstane-3alpha,17beta-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0004
-
glycolithocholic acid
cofactor NADP+
Mus musculus
1.1.1.209
0.0005
-
5beta-pregnane-3alpha,20alpha-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0007
-
5beta-pregnane-3alpha-ol-20-one
cofactor NADP+
Mus musculus
1.1.1.209
0.004
-
NADP+
-
Mus musculus
1.1.1.209
0.16
-
(R)-1-indanol
cofactor NADP+
Mus musculus
1.1.1.209
0.26
-
NAD+
-
Mus musculus
1.1.1.209
0.77
-
trans-benzene dihydrodiol
cofactor NADP+
Mus musculus
1.1.1.209
1.99
-
cis-benzene dihydrodiol
cofactor NADP+
Mus musculus
1.1.1.209
2.23
-
cyclohex-2-ene-1-ol
cofactor NADP+
Mus musculus
1.1.1.209
2.67
-
Naphthalene dihydrodiol
cofactor NADP+
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.209
34000
-
gel filtration
Mus musculus
1.1.1.209
39000
-
1 * 39000, SDS-PAGE
Mus musculus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.209
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.209
Q-Sepharose, Matrex Red A, HA-Ultrogel, Sephadex G-100, two enzyme preparations DD1 and DD2 with almost identical properties, products of post-translational or post-mortem modification rather than isoenzymes
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.209
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.209
0.27
-
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.209
(R)-1-indanol + NADP+
37% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-indanone + NADPH
-
286234
Mus musculus
?
1.1.1.209
(S)-1-indanole + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-indanone + NADPH
-
286234
Mus musculus
?
1.1.1.209
1-acenaphthenol + NADP+
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-acenaphthenone + NADPH
-
286234
Mus musculus
?
1.1.1.209
4-nitrobenzaldehyde + NADPH
58% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
4-nitrobenzyl alcohol + NADP+
-
286234
Mus musculus
?
1.1.1.209
5alpha-androstane-3alpha,17beta-diol + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-androstane-17beta-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnan-3alpha,20alpha-diol + NADP+
6% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnan-20alpha-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnan-3alpha,21-diol-20-one + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnan-21alpha-ol-3,20-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnane-21-ol-3,20-dione + NADPH
75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnane-3alpha,21-diol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnane-3,20-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnane-3alpha-ol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstan-17beta-ol-3-one + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstan-17beta,3alpha-diol + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3,17-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstan-3alpha-ol-17-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3alpha,17beta-diol + NADP+
11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstane-17beta-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3alpha-ol-17-one + NADP+
9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstane-3,17-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnan-3alpha,20alpha-diol + NADP+
26% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnan-3-one-20alpha-ol + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnan-3alpha-ol-20-one + NADP+
19% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnan-3,20-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnane-3,20-dione + NADPH + H+
21% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnane-3alpha-ol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
acenaphthenequinone + NADPH
44% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
acenaphthenehydroquinone + NADP+
-
286234
Mus musculus
?
1.1.1.209
androst-4-ene-3,17-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
androst-4-ene-3alpha-ol-17-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
camphorquinone + NADPH + H+
52% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
camphorhydroquinone + NADP+
-
286234
Mus musculus
?
1.1.1.209
cis-1,2-dihydroxy-3,5-cyclohexadiene + NADP+
60% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
catechol + NADPH
-
286234
Mus musculus
?
1.1.1.209
cyclohex-2-ene-1-ol + NADP+
9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
cyclohex-2-en-1-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
cyclohexanone + NADPH
16% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
cyclohexanol + NADP+
-
286234
Mus musculus
?
1.1.1.209
epitestosterone + NADP+
11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
testosterone + NADPH + H+
-
286234
Mus musculus
?
1.1.1.209
glycolithocholic acid + NADP+
56% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
N-[(5beta)-3,24-dioxocholan-24-yl]glycine + NADPH
-
286234
Mus musculus
?
1.1.1.209
naphthalene dihydrodiol + NADP+
49% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
naphthacatechol + NADPH
-
286234
Mus musculus
?
1.1.1.209
pyridine-4-ol + NADP+
75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
3H-pyridine-4-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
tetrahydrodexycorticosterone + NAD+
-
286234
Mus musculus
?
-
-
-
r
1.1.1.209
trans-1,2-dihydroxy-3,5-cyclohexadiene + NADP+
-
286234
Mus musculus
catechol + NADPH
-
286234
Mus musculus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.209
monomer
1 * 39000, SDS-PAGE
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.209
6
6.8
5beta-pregnane-3,20-dione as substrate, cofactor NADPH
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.209
17beta-estradiol
5 mM, 77% inhibition
Mus musculus
1.1.1.209
5alpha-Androstan-3beta-ol-17-one
1 mM, 80% inhibition
Mus musculus
1.1.1.209
5alpha-pregnan-3beta-ol-21-one
1 mM, 66% inhibition
Mus musculus
1.1.1.209
5beta-androstan-3beta-ol-17-one
1 mM, 91% inhibition
Mus musculus
1.1.1.209
5beta-Pregnan-3beta-ol-20-one
1 mM, 80% inhibition
Mus musculus
1.1.1.209
Barbital
100 mM, 11% inhibition
Mus musculus
1.1.1.209
Dienstrol
1 mM, 22% inhibition
Mus musculus
1.1.1.209
Hexestrol
1 mM, 77% inhibition
Mus musculus
1.1.1.209
Stilbestrol
10 mM, 75% inhibition
Mus musculus
1.1.1.209
testosterone
1 mM, 48% inhibition
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.209
0.0001
-
Epitestosterone
cofactor NADP+
Mus musculus
1.1.1.209
0.0001
-
5beta-androstane-3alpha,17beta-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0003
-
5beta-androstane-3alpha,17beta-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0004
-
glycolithocholic acid
cofactor NADP+
Mus musculus
1.1.1.209
0.0005
-
5beta-pregnane-3alpha,20alpha-diol
cofactor NADP+
Mus musculus
1.1.1.209
0.0007
-
5beta-pregnane-3alpha-ol-20-one
cofactor NADP+
Mus musculus
1.1.1.209
0.004
-
NADP+
-
Mus musculus
1.1.1.209
0.16
-
(R)-1-indanol
cofactor NADP+
Mus musculus
1.1.1.209
0.26
-
NAD+
-
Mus musculus
1.1.1.209
0.77
-
trans-benzene dihydrodiol
cofactor NADP+
Mus musculus
1.1.1.209
1.99
-
cis-benzene dihydrodiol
cofactor NADP+
Mus musculus
1.1.1.209
2.23
-
cyclohex-2-ene-1-ol
cofactor NADP+
Mus musculus
1.1.1.209
2.67
-
Naphthalene dihydrodiol
cofactor NADP+
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.209
34000
-
gel filtration
Mus musculus
1.1.1.209
39000
-
1 * 39000, SDS-PAGE
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.209
Q-Sepharose, Matrex Red A, HA-Ultrogel, Sephadex G-100, two enzyme preparations DD1 and DD2 with almost identical properties, products of post-translational or post-mortem modification rather than isoenzymes
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.209
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.209
0.27
-
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.209
(R)-1-indanol + NADP+
37% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-indanone + NADPH
-
286234
Mus musculus
?
1.1.1.209
(S)-1-indanole + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-indanone + NADPH
-
286234
Mus musculus
?
1.1.1.209
1-acenaphthenol + NADP+
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
1-acenaphthenone + NADPH
-
286234
Mus musculus
?
1.1.1.209
4-nitrobenzaldehyde + NADPH
58% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
4-nitrobenzyl alcohol + NADP+
-
286234
Mus musculus
?
1.1.1.209
5alpha-androstane-3alpha,17beta-diol + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-androstane-17beta-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnan-3alpha,20alpha-diol + NADP+
6% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnan-20alpha-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnan-3alpha,21-diol-20-one + NADP+
7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnan-21alpha-ol-3,20-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnane-21-ol-3,20-dione + NADPH
75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnane-3alpha,21-diol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5alpha-pregnane-3,20-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5alpha-pregnane-3alpha-ol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstan-17beta-ol-3-one + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstan-17beta,3alpha-diol + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3,17-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstan-3alpha-ol-17-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3alpha,17beta-diol + NADP+
11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstane-17beta-ol-3-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-androstane-3alpha-ol-17-one + NADP+
9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-androstane-3,17-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnan-3alpha,20alpha-diol + NADP+
26% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnan-3-one-20alpha-ol + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnan-3alpha-ol-20-one + NADP+
19% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnan-3,20-dione + NADPH
-
286234
Mus musculus
?
1.1.1.209
5beta-pregnane-3,20-dione + NADPH + H+
21% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
5beta-pregnane-3alpha-ol-20-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
acenaphthenequinone + NADPH
44% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
acenaphthenehydroquinone + NADP+
-
286234
Mus musculus
?
1.1.1.209
androst-4-ene-3,17-dione + NADPH
10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
androst-4-ene-3alpha-ol-17-one + NADP+
-
286234
Mus musculus
?
1.1.1.209
camphorquinone + NADPH + H+
52% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
camphorhydroquinone + NADP+
-
286234
Mus musculus
?
1.1.1.209
cis-1,2-dihydroxy-3,5-cyclohexadiene + NADP+
60% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
catechol + NADPH
-
286234
Mus musculus
?
1.1.1.209
cyclohex-2-ene-1-ol + NADP+
9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
cyclohex-2-en-1-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
cyclohexanone + NADPH
16% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
cyclohexanol + NADP+
-
286234
Mus musculus
?
1.1.1.209
epitestosterone + NADP+
11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
testosterone + NADPH + H+
-
286234
Mus musculus
?
1.1.1.209
glycolithocholic acid + NADP+
56% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
N-[(5beta)-3,24-dioxocholan-24-yl]glycine + NADPH
-
286234
Mus musculus
?
1.1.1.209
naphthalene dihydrodiol + NADP+
49% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
naphthacatechol + NADPH
-
286234
Mus musculus
?
1.1.1.209
pyridine-4-ol + NADP+
75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene
286234
Mus musculus
3H-pyridine-4-one + NADPH
-
286234
Mus musculus
?
1.1.1.209
tetrahydrodexycorticosterone + NAD+
-
286234
Mus musculus
?
-
-
-
r
1.1.1.209
trans-1,2-dihydroxy-3,5-cyclohexadiene + NADP+
-
286234
Mus musculus
catechol + NADPH
-
286234
Mus musculus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.209
monomer
1 * 39000, SDS-PAGE
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.209
6
6.8
5beta-pregnane-3,20-dione as substrate, cofactor NADPH
Mus musculus