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Literature summary extracted from

  • Brown, H.M.; Purves, W.K.
    Indoleacetaldehyde reductase of Cucumis sativus L. Kinetic properties and role in auxin biosynthesis (1980), Plant Physiol., 65, 107-113.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.191 NaCl activation up to 0.1 M Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.190 Cd2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.190 Cu2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.190 EDTA strong inhibition at 10 mM Cucumis sativus
1.1.1.190 Hg2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.190 iodoacetic acid 62% inhibition at 10 mM Cucumis sativus
1.1.1.190 KCN complete inhibition at 1 mM Cucumis sativus
1.1.1.190 N-ethylmaleimide 8§% inhibition at 1 mM Cucumis sativus
1.1.1.190 NaCl uncompetitive inhibition with respect to NADH, competitive inhibition with respect to indole-3-acetaldehyde Cucumis sativus
1.1.1.190 NaN3 slight inhibition at 10 mM Cucumis sativus
1.1.1.191 Cd2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.191 Cu2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.191 EDTA 40% inhibition at 1 mM Cucumis sativus
1.1.1.191 Hg2+ complete inhibition at 2 mM Cucumis sativus
1.1.1.191 iodoacetate 22% inhibition at 1 mM Cucumis sativus
1.1.1.191 KCN 73% inhibition at 1 mM Cucumis sativus
1.1.1.191 N-ethylmaleimide 46% inhibition at 1 mM Cucumis sativus
1.1.1.191 NADPH inhibition at high concentrations Cucumis sativus
1.1.1.191 NaF 35% inhibition at 1 mM Cucumis sativus
1.1.1.191 NaN3 slight inhibition at 1 mM Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.190 0.14
-
 NADH marked function of the NaCl concentration Cucumis sativus
1.1.1.190 0.5
-
 Indole-3-acetaldehyde marked function of the NaCl concentration Cucumis sativus
1.1.1.191 0.011
-
 NADPH
-
 Cucumis sativus
1.1.1.191 0.125
-
 Indole-3-acetaldehyde
-
 Cucumis sativus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.191 Ca2+ 29% activation at 2 mM Cucumis sativus
1.1.1.191 Mg2+ 29% activation at 2 mM Cucumis sativus
1.1.1.191 Mn2+ 39% activation at 2 mM Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.190 32000
-
 gel filtration Cucumis sativus
1.1.1.191 32000
-
 gel filtration Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.190 indole-3-acetaldehyde + NADH Cucumis sativus involved in auxin biosynthetic pathway indole-3-ethanol + NAD+
-
 ?
1.1.1.191 indole-3-acetaldehyde + NADPH Cucumis sativus
-
 indole-3-ethanol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.190 Cucumis sativus
-
-
-
1.1.1.191 Cucumis sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.190 partial Cucumis sativus
1.1.1.191 partial Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.190 seedling
-
 Cucumis sativus
-
1.1.1.191 seed
-
 Cucumis sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.190 indole-3-acetaldehyde + NADH involved in auxin biosynthetic pathway Cucumis sativus indole-3-ethanol + NAD+
-
 ?
1.1.1.191 indole-3-acetaldehyde + NADPH
-
 Cucumis sativus indole-3-ethanol + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.190 NAD+
-
 Cucumis sativus
1.1.1.191 NADP+
-
 Cucumis sativus