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Literature summary extracted from

  • Asada, Y.; Aoki, S.; Ishikura, S.; Usami, N.; Hara, A.
    Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function (2000), Biochem. Biophys. Res. Commun., 278, 333-337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.179 additional information
-
 Macaca fuscata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.179 cDNA subcloned in expression vector pRset, overexpression in Escherichia coli Macaca fuscata
1.3.1.20 expression of wild-type and mutants, H79Q and Y180F, in Escherichia coli Macaca fuscata

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.179 H79Q increase in Km more than 25fold, increase in Kd more than 7fold, with high ionic strength Km increased by 190fold and inhibited the enzyme Macaca fuscata
1.1.1.179 Y180F almost inactive, high affinity for NADP(H) Macaca fuscata
1.3.1.20 H79Q site-directed mutagenesis, moderate activity with 190fold increase in Km-value, inhibition by MgCl2 and potassium phosphate Macaca fuscata
1.3.1.20 additional information sequence analysis reveals high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes Macaca fuscata
1.3.1.20 Y180F site-directed mutagenesis, very low activity Macaca fuscata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.179 high salt concentration recombinant H79Q mutant Macaca fuscata
1.3.1.20 MgCl2 inhibition of mutant enzymes H79Q and Y180F Macaca fuscata
1.3.1.20 phosphate buffer inhibitionn of mutant enzymes H79Q and Y180F Macaca fuscata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.179 additional information
-
 additional information phosphate buffer increases the Km-value, especially for mutant H79Q, compared with Tris buffer Macaca fuscata
1.1.1.179 0.0006
-
 NADPH camphorquinone Macaca fuscata
1.1.1.179 0.0006
-
 NADP+ D-xylose Macaca fuscata
1.1.1.179 0.014
-
 NADP+ D-xylose, mutant H79Q Macaca fuscata
1.1.1.179 0.028
-
 NADPH camphorquinone, mutant H79Q Macaca fuscata
1.1.1.179 0.1
-
 Camphorquinone
-
 Macaca fuscata
1.1.1.179 0.51
-
 Camphorquinone mutant H79Q Macaca fuscata
1.1.1.179 4.4
-
 D-xylose
-
 Macaca fuscata
1.1.1.179 67
-
 D-xylose mutant H79Q Macaca fuscata
1.3.1.20 0.1
-
 Camphorquinone NADPH Macaca fuscata
1.3.1.20 0.51
-
 Camphorquinone mutant H79Q, NADPH Macaca fuscata
1.3.1.20 0.55
-
 NADP+ phosphate buffer 0.1 M Macaca fuscata
1.3.1.20 0.56
-
 NADPH
-
 Macaca fuscata
1.3.1.20 2.1
-
 NADP+ Tris buffer 25 mM Macaca fuscata
1.3.1.20 4.4
-
 D-xylose NADP+, 25 mM Tris buffer 25 mM Macaca fuscata
1.3.1.20 6.3
-
 D-xylose NADP+, 0.1 M phosphate buffer Macaca fuscata
1.3.1.20 14
-
 NADP+ mutant H79Q, phosphate buffer 0.1 M Macaca fuscata
1.3.1.20 28
-
 NADPH mutant H79Q Macaca fuscata
1.3.1.20 67
-
 D-xylose mutant H79Q, NADP+, 0.1 M phosphate buffer Macaca fuscata
1.3.1.20 213
-
 D-xylose mutant H79Q, NADP+, Tris buffer 25 mM Macaca fuscata
1.3.1.20 402
-
 NADP+ mutant H70Q, Tris buffer 25 mM Macaca fuscata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.179 36000
-
 2 * 36000, SDS-PAGE Macaca fuscata
1.1.1.179 70000
-
 about, gel filtration Macaca fuscata
1.3.1.20 36000
-
 2 * 36000, SDS-PAGE Macaca fuscata
1.3.1.20 70000
-
 gel filtration Macaca fuscata

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.179 Macaca fuscata
-
-
-
1.3.1.20 Macaca fuscata
-
 japanese monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.179 recombinant enzyme and mutants Y180F, H79Q Macaca fuscata
1.3.1.20 wild-type and mutant protein recombinant from Escherichia coli Macaca fuscata

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ identical with D-xylose dehydrogenase EC 1.1.1.179 Macaca fuscata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.179 kidney
-
 Macaca fuscata
-
1.3.1.20 kidney
-
 Macaca fuscata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.179 5.3 5.5 wild-type and mutant H79Q Macaca fuscata
1.1.1.179 18
-
 substrate camphorquinone, mutant H79Q Macaca fuscata
1.1.1.179 33
-
 substrate camphorquinone Macaca fuscata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.179 camphorquinone + NADPH
-
 Macaca fuscata (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
 ?
1.1.1.179 D-xylose + NADP+
-
 Macaca fuscata D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Macaca fuscata ?
-
 ?
1.3.1.20 camphorquinone + NAD(P)H
-
 Macaca fuscata (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
 ?
1.3.1.20 D-xylose + NADP+
-
 Macaca fuscata D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.3.1.20 additional information monomeric forms are identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.50 and provide a broad substrate specificity, while dimeric forms are identical with D-xylose dehydrogenase EC 1.1.1.179 and are active with trans-dihydrodiols and dicarbonyl compounds Macaca fuscata additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.179 dimer 2 * 36000, SDS-PAGE Macaca fuscata
1.3.1.20 dimer 2 * 36000, SDS-PAGE Macaca fuscata

Synonyms

EC Number Synonyms Comment Organism
1.3.1.20 DD
-
 Macaca fuscata
1.3.1.20 dihydrodiol dehydrogenase
-
 Macaca fuscata
1.3.1.20 More sequence analysis revealed high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes Macaca fuscata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.179 NADP+
-
 Macaca fuscata
1.3.1.20 NADP+
-
 Macaca fuscata
1.3.1.20 NADPH
-
 Macaca fuscata