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Literature summary extracted from

  • Nakano, Y.; Suzuki, N.; Yoshida, Y.; Nezu, T.; Yamashita, Y.; Koga, T.
    Thymidine diphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans (2000), J. Biol. Chem., 275, 6806-6812.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.133 overexpression in Escherichia coli DH5alpha, ER2566, BL21(DE3) Aggregatibacter actinomycetemcomitans
1.1.1.339 expression in Escherichia coli Aggregatibacter actinomycetemcomitans
1.1.1.344 expressed in Escherichia coli BL21 cells Aggregatibacter actinomycetemcomitans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.133 31900
-
 Y4 rmlD gene product, SDS-PAGE Aggregatibacter actinomycetemcomitans
1.1.1.133 32400
-
 NCTC 9710 rmlD gene product, SDS-PAGE Aggregatibacter actinomycetemcomitans
1.1.1.339 30700
-
 x * 30700, SDS-PAGE Aggregatibacter actinomycetemcomitans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.133 Aggregatibacter actinomycetemcomitans
-
 Y4, NCTC 9710
-
1.1.1.133 Escherichia coli
-
 O45
-
1.1.1.133 Escherichia coli O45
-
 O45
-
1.1.1.339 Aggregatibacter actinomycetemcomitans
-
 NCTC 9710
-
1.1.1.344 Aggregatibacter actinomycetemcomitans
-
-
-
1.1.1.344 Aggregatibacter actinomycetemcomitans NCTC 9710
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.133 chromatography Aggregatibacter actinomycetemcomitans
1.1.1.344 chitin bead chromatography and amylose column chromatography Aggregatibacter actinomycetemcomitans

Storage Stability

EC Number Storage Stability Organism
1.1.1.133 -20°C, purified protein, 50% glycerol Aggregatibacter actinomycetemcomitans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
-
 Aggregatibacter actinomycetemcomitans dTDP-L-rhamnose + NADP+
-
 ?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
-
 Aggregatibacter actinomycetemcomitans NCTC 9710 dTDP-L-rhamnose + NADP+
-
 ?
1.1.1.339 dTDP-4-dehydro-6-deoxy-beta-L-mannose + NADH + H+
-
 Aggregatibacter actinomycetemcomitans dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?
1.1.1.339 dTDP-4-dehydro-6-deoxy-beta-L-mannose + NADH + H+
-
 Aggregatibacter actinomycetemcomitans NCTC 9710 dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?
1.1.1.344 dTDP-6-deoxy-L-lyxo-4-hexulose + NADH + H+
-
 Aggregatibacter actinomycetemcomitans dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?
1.1.1.344 dTDP-6-deoxy-L-lyxo-4-hexulose + NADH + H+
-
 Aggregatibacter actinomycetemcomitans NCTC 9710 dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.339 ? x * 30700, SDS-PAGE Aggregatibacter actinomycetemcomitans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
 Aggregatibacter actinomycetemcomitans
1.1.1.133 thymidine diphosphate-6-deoxy-L-talose
-
 Aggregatibacter actinomycetemcomitans
1.1.1.339 dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
 Aggregatibacter actinomycetemcomitans
1.1.1.339 tll
-
 Aggregatibacter actinomycetemcomitans
1.1.1.344 dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
 Aggregatibacter actinomycetemcomitans
1.1.1.344 thymidine diphosphate-6-deoxy-L-lyxo-4-hexulose reductase
-
 Aggregatibacter actinomycetemcomitans
1.1.1.344 tll
-
 Aggregatibacter actinomycetemcomitans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.133 NADPH
-
 Escherichia coli
1.1.1.133 NADPH
-
 Aggregatibacter actinomycetemcomitans
1.1.1.344 NADH
-
 Aggregatibacter actinomycetemcomitans

General Information

EC Number General Information Comment Organism
1.1.1.339 physiological function enzyme is part of the pathway leading to the unusual sugar, 6-deoxy-L-talose, which is a conctituent of cell wall components in some bacteria Aggregatibacter actinomycetemcomitans