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Literature summary extracted from

  • Preiss, J.
    Sugar nucleotide reactions in Arthrobacter. II. Biosynthesis of guanosine diphosphomannuronate (1964), J. Biol. Chem., 239, 3127-3132.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.132 0.017
-
 GDP-mannose
-
 Arthrobacter sp.
1.1.1.132 0.084
-
 deoxy-GDP-D-mannose
-
 Arthrobacter sp.
1.1.1.132 0.26
-
 NAD+ cosubstrate 0.3 mM GDP-mannose Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.132 Arthrobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.132 ammonium sulfate fractionation, affinity chromatography Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.132 0.245
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.132 Deoxy-GDP-D-mannose + 2 NAD+ + H2O
-
 Arthrobacter sp. deoxy-GDP-D-mannuronate + 2 NADH + 2 H+
-
 r
1.1.1.132 deoxy-GDP-D-mannose + NAD+ + H2O
-
 Arthrobacter sp. deoxy-GDP-D-mannuronate + NADH
-
 ?
1.1.1.132 GDP-mannose + NAD+ + H2O
-
 Arthrobacter sp. GDP-mannuronate + NADH
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.132 8.2
-
 Tris buffer Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.132 NAD+
-
 Arthrobacter sp.
1.1.1.132 NADH
-
 Arthrobacter sp.