EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.132 | ATP | 26% inhibition at 1 mM | Pseudomonas aeruginosa | |
1.1.1.132 | D-maltose | 17% inhibition at 1 mM | Pseudomonas aeruginosa | |
1.1.1.132 | GDP-D-glucose | 25% inhibition at 1 mM | Pseudomonas aeruginosa | |
1.1.1.132 | GMP | competitive, 52% inhibition at 1 mM; competitive, most potent | Pseudomonas aeruginosa | |
1.1.1.132 | iodacetamide | 40% inactivation within 30 min, complete reactivation by dithiothreitol | Pseudomonas aeruginosa | |
1.1.1.132 | iodoacetamide | 40% inactivation after 30 min preincubation | Pseudomonas aeruginosa | |
1.1.1.132 | maltose | - |
Pseudomonas aeruginosa | |
1.1.1.132 | additional information | full reactivation by dithiothreitol (25 mM) after p-hydroxymercuribenzoate or iodoacetamide inactivation | Pseudomonas aeruginosa | |
1.1.1.132 | p-hydroxymercuribenzoate | 96% inactivation after 5 min preincubation; 96% inactivation within 5 min, complete reactivation by dithiothreitol | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.132 | 0.0149 | - |
GDP-D-mannose | - |
Pseudomonas aeruginosa | |
1.1.1.132 | 0.0149 | - |
GDP-D-mannose | 24°C, pH 8.0 | Pseudomonas aeruginosa | |
1.1.1.132 | 0.185 | - |
NAD+ | cosubstrate GDP-D-mannose | Pseudomonas aeruginosa | |
1.1.1.132 | 0.185 | - |
NAD+ | 24°C, pH 8.0 | Pseudomonas aeruginosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.132 | soluble | - |
Pseudomonas aeruginosa | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.132 | 48000 | - |
6 * 48000, SDS-PAGE | Pseudomonas aeruginosa |
1.1.1.132 | 290000 | - |
gel filtration | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.132 | additional information | Pseudomonas aeruginosa | key enzyme in alginate biosynthesis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.132 | Pseudomonas aeruginosa | - |
- |
- |
1.1.1.132 | Pseudomonas aeruginosa | - |
mucoid strain 8821 | - |
1.1.1.132 | Pseudomonas aeruginosa | - |
enzyme overproduced using a plasmid vector containing algD under control of the tac promoter | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.132 | pH/heat treatment acetone precipitation HPLC gel filtration chromatography | Pseudomonas aeruginosa |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.1.1.132 | inactivation by iodacetamide or p-hydroxymercuribenzoate is completely reversible | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.132 | 1.068 | - |
- |
Pseudomonas aeruginosa |
1.1.1.132 | 1068 | - |
24°C, pH 8.0 | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.132 | GDP-D-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-D-mannuronate + NADH | - |
? | |
1.1.1.132 | GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
? | |
1.1.1.132 | additional information | key enzyme in alginate biosynthesis | Pseudomonas aeruginosa | ? | - |
? | |
1.1.1.132 | additional information | no substrate: D-mannose, UDP-D-mannose, UDP-D-glucose, TDP-D-glucose, CDP-D-glucose | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.132 | hexamer | 6 * 48000, SDS-PAGE | Pseudomonas aeruginosa |
1.1.1.132 | More | N-terminal amino acid sequence | Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.132 | 50 | - |
- |
Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.132 | 50 | - |
above, 2-3 min, loss of activity | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.132 | 7.7 | - |
- |
Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.132 | NAD+ | - |
Pseudomonas aeruginosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.132 | 0.0227 | - |
GMP | 24°C, pH 8.0 | Pseudomonas aeruginosa |