Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aspen, A.J.; Jakoby, W.B.
    L-threonic acid dehydrogenase: Purification and properties (1964), J. Biol. Chem., 239, 710-713.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.129 hydrazine inhibitory at 100 mM Pseudomonas sp.
1.1.1.129 hydroxylamine inhibitory at 100 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.129 L-threonate + NAD+ Pseudomonas sp.
-
 3-dehydro-L-threonate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.129 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.129 100fold purification, calcium phosphate gel Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.129 cell culture inducible by growing in L-threonate-containing medium Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.129 124
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.129 -15°C, 30 mM Tris-HCl, pH 7.5, 5 mM 2-mercaptoethanol, 0.5 M NaCl, 44% retaining activity after 8 weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.129 L-threonate + NAD+
-
 Pseudomonas sp. 3-dehydro-L-threonate + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.129 10.8
-
 oxidation reaction Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.129 8.9 11.5 50% of maximal activity at pH 8.9 and pH 11.5 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.129 NAD+ cannot be replaced by NADP+ Pseudomonas sp.