BRENDA - Enzyme Database

Enzymatic reduction of alkyl and acyl derivatives of dihydroxyacetone phosphate by reduced pyridine nucleotides

LaBelle, E.F.; Hajra, A.K.; J. Biol. Chem. 247, 5825-5834 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Cavia porcellus
1.1.1.101
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
1.1.1.101
freeze-thawing, stable after several cycles
Cavia porcellus
1.1.1.101
freeze-thawing, stable after several cycles
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.005
-
NADPH
cosubstrate hexadecyldihydroxyacetone phosphate, brain
Rattus norvegicus
1.1.1.101
0.007
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, brain
Rattus norvegicus
1.1.1.101
0.01
-
NADPH
ascites microsomes
Cavia porcellus
1.1.1.101
0.01
-
NADPH
ascites microsomes
Rattus norvegicus
1.1.1.101
0.05
-
NADPH
liver mitochondria
Cavia porcellus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.101
Cavia porcellus
-
-
-
1.1.1.101
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
adipose tissue
-
Rattus norvegicus
-
1.1.1.101
ascites tumor cell
grown in the abdomen of swiss white mice
Cavia porcellus
-
1.1.1.101
ascites tumor cell
grown in the abdomen of swiss white mice
Rattus norvegicus
-
1.1.1.101
brain
-
Rattus norvegicus
-
1.1.1.101
heart
-
Rattus norvegicus
-
1.1.1.101
kidney
-
Rattus norvegicus
-
1.1.1.101
liver
-
Rattus norvegicus
-
1.1.1.101
liver
-
Cavia porcellus
-
1.1.1.101
lung
-
Cavia porcellus
-
1.1.1.101
spleen
-
Cavia porcellus
-
1.1.1.101
testis
-
Cavia porcellus
-
1.1.1.101
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.101
100
-
complete inactivation after 10 min
Cavia porcellus
1.1.1.101
100
-
complete inactivation after 10 min
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.101
6.5
7.5
broad optimum
Rattus norvegicus
1.1.1.101
6.5
7.5
broad optimum
Cavia porcellus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
at high concentrations, rat liver
Rattus norvegicus
1.1.1.101
NADH
-
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Cavia porcellus
1.1.1.101
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
at high concentrations, rat liver
Rattus norvegicus
1.1.1.101
NADH
-
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.101
freeze-thawing, stable after several cycles
Cavia porcellus
1.1.1.101
freeze-thawing, stable after several cycles
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.005
-
NADPH
cosubstrate hexadecyldihydroxyacetone phosphate, brain
Rattus norvegicus
1.1.1.101
0.007
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, brain
Rattus norvegicus
1.1.1.101
0.01
-
NADPH
ascites microsomes
Cavia porcellus
1.1.1.101
0.01
-
NADPH
ascites microsomes
Rattus norvegicus
1.1.1.101
0.05
-
NADPH
liver mitochondria
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
adipose tissue
-
Rattus norvegicus
-
1.1.1.101
ascites tumor cell
grown in the abdomen of swiss white mice
Cavia porcellus
-
1.1.1.101
ascites tumor cell
grown in the abdomen of swiss white mice
Rattus norvegicus
-
1.1.1.101
brain
-
Rattus norvegicus
-
1.1.1.101
heart
-
Rattus norvegicus
-
1.1.1.101
kidney
-
Rattus norvegicus
-
1.1.1.101
liver
-
Rattus norvegicus
-
1.1.1.101
liver
-
Cavia porcellus
-
1.1.1.101
lung
-
Cavia porcellus
-
1.1.1.101
spleen
-
Cavia porcellus
-
1.1.1.101
testis
-
Cavia porcellus
-
1.1.1.101
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.101
100
-
complete inactivation after 10 min
Cavia porcellus
1.1.1.101
100
-
complete inactivation after 10 min
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.101
6.5
7.5
broad optimum
Rattus norvegicus
1.1.1.101
6.5
7.5
broad optimum
Cavia porcellus