BRENDA - Enzyme Database

Purification and partial characterization of alcohol dehydrogenase from wheat

Langston, P.J.; Hart, G.E.; Pace, C.N.; Arch. Biochem. Biophys. 196, 611-618 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
2-mercaptoethanol
-
Triticum monococcum
1.1.1.1
glutathione
-
Triticum monococcum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.1
-
NAD+
-
Triticum monococcum
1.1.1.1
12
-
ethanol
-
Triticum monococcum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
58000
-
2 * 58000, SDS-PAGE
Triticum monococcum
1.1.1.1
116000
-
gel filtration
Triticum monococcum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Triticum monococcum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.1
-
Triticum monococcum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.1
55900
-
-
Triticum monococcum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
2-methylpropan-1-ol + NAD+
-
285633
Triticum monococcum
2-methyl-propan-1-one + NADH
-
-
-
-
1.1.1.1
3-methylbutanol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285633
Triticum monococcum
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285633
Triticum monococcum
cyclohexanone + NADH
-
-
-
?
1.1.1.1
cyclopentanol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
D-glucitol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285633
Triticum monococcum
acetaldehyde + NADH
-
-
-
-
1.1.1.1
hexan-1-ol + NAD+
-
285633
Triticum monococcum
n-hexanal + NADH
-
-
-
?
1.1.1.1
pentanol + NAD+
-
285633
Triticum monococcum
n-pentanal + NADH
-
-
-
?
1.1.1.1
propanol + NADH
-
285633
Triticum monococcum
propionaldehyde + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 58000, SDS-PAGE
Triticum monococcum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Triticum monococcum
1.1.1.1
NADH
-
Triticum monococcum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Triticum monococcum
1.1.1.1
NADH
-
Triticum monococcum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
2-mercaptoethanol
-
Triticum monococcum
1.1.1.1
glutathione
-
Triticum monococcum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.1
-
NAD+
-
Triticum monococcum
1.1.1.1
12
-
ethanol
-
Triticum monococcum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
58000
-
2 * 58000, SDS-PAGE
Triticum monococcum
1.1.1.1
116000
-
gel filtration
Triticum monococcum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
-
Triticum monococcum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.1
55900
-
-
Triticum monococcum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
2-methylpropan-1-ol + NAD+
-
285633
Triticum monococcum
2-methyl-propan-1-one + NADH
-
-
-
-
1.1.1.1
3-methylbutanol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285633
Triticum monococcum
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285633
Triticum monococcum
cyclohexanone + NADH
-
-
-
?
1.1.1.1
cyclopentanol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
D-glucitol + NAD+
-
285633
Triticum monococcum
? + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285633
Triticum monococcum
acetaldehyde + NADH
-
-
-
-
1.1.1.1
hexan-1-ol + NAD+
-
285633
Triticum monococcum
n-hexanal + NADH
-
-
-
?
1.1.1.1
pentanol + NAD+
-
285633
Triticum monococcum
n-pentanal + NADH
-
-
-
?
1.1.1.1
propanol + NADH
-
285633
Triticum monococcum
propionaldehyde + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 58000, SDS-PAGE
Triticum monococcum