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Literature summary extracted from

  • Hegeman, G.D.; Rosenberg, E.Y.; Kenyon, G.L.
    Mandelic acid racemase from Pseudomonas putida. Purification and properties of the enzyme (1970), Biochemistry, 9, 4029-4036.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.2.2 benzoate weak Pseudomonas putida
5.1.2.2 Diphenylacetic acid
-
 Pseudomonas putida
5.1.2.2 DL-Atrolactate
-
 Pseudomonas putida
5.1.2.2 DL-beta-Phenyllactate
-
 Pseudomonas putida
5.1.2.2 iodoacetamide weak noncompetitive inhibitor Pseudomonas putida
5.1.2.2 NEM weak noncompetitive inhibitor Pseudomonas putida
5.1.2.2 p-chloromercuribenzoate weak noncompetitive inhibitor Pseudomonas putida
5.1.2.2 phenylacetate
-
 Pseudomonas putida
5.1.2.2 Phenylmercaptoacetate
-
 Pseudomonas putida
5.1.2.2 Phenyloxyacetate
-
 Pseudomonas putida
5.1.2.2 phosphate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.2.2 0.093
-
 D-mandelate
-
 Pseudomonas putida
5.1.2.2 0.1
-
 D(-)-mandelate 4-chloro-D-mandelate Pseudomonas putida
5.1.2.2 0.256
-
 4-Bromo-D-mandelate
-
 Pseudomonas putida
5.1.2.2 0.29
-
 4-Hydroxy-D-mandelate
-
 Pseudomonas putida
5.1.2.2 0.33
-
 4-Methoxy-D-mandelate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.2.2 Divalent metal ions increase the affinity of the enzyme for the substrate. No absolute requirement Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.2.2 200000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.2.2 D-mandelate Pseudomonas putida inducible enzyme L-mandelate
-
 ?
5.1.2.2 D-mandelate Pseudomonas putida A.3.12 inducible enzyme L-mandelate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.2.2 Pseudomonas putida
-
 A.3.12
-
5.1.2.2 Pseudomonas putida A.3.12
-
 A.3.12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.2.2
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.2.2 195
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.2.2 4-bromo-D-mandelate
-
 Pseudomonas putida 4-bromo-L-mandelate
-
 ?
5.1.2.2 4-bromo-D-mandelate
-
 Pseudomonas putida A.3.12 4-bromo-L-mandelate
-
 ?
5.1.2.2 4-chloro-D-mandelate
-
 Pseudomonas putida 4-chloro-L-mandelate
-
 ?
5.1.2.2 4-chloro-D-mandelate
-
 Pseudomonas putida A.3.12 4-chloro-L-mandelate
-
 ?
5.1.2.2 4-hydroxy-D-mandelate
-
 Pseudomonas putida 4-hydroxy-L-mandelate
-
 ?
5.1.2.2 4-hydroxy-D-mandelate
-
 Pseudomonas putida A.3.12 4-hydroxy-L-mandelate
-
 ?
5.1.2.2 4-methoxy-D-mandelate
-
 Pseudomonas putida 4-methoxy-L-mandelate
-
 ?
5.1.2.2 D-mandelate
-
 Pseudomonas putida L-mandelate
-
 ?
5.1.2.2 D-mandelate inducible enzyme Pseudomonas putida L-mandelate
-
 ?
5.1.2.2 D-mandelate
-
 Pseudomonas putida A.3.12 L-mandelate
-
 ?
5.1.2.2 D-mandelate inducible enzyme Pseudomonas putida A.3.12 L-mandelate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.2.2 7.5 8
-
 Pseudomonas putida