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Literature summary extracted from

  • Kern, D.; Potier, S.; Lapointe, J.; Boulanger, Y.
    The glutaminyl-transfer RNA synthetase of Escherichia coli. Purification, structure and function relationship (1980), Biochim. Biophys. Acta, 607, 65-80.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.18 spermine significant stimulation of ATP-diphosphate exchange and aminoacylation in presence of limited MgCl2 concentrations, cannot totally replace Mg2+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.18 5,5'-dithiobis(2-nitrobenzoate)
-
 Escherichia coli
6.1.1.18 p-hydroxymercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.18 0.0002
-
 tRNAGln
-
 Escherichia coli
6.1.1.18 0.15
-
 Gln
-
 Escherichia coli
6.1.1.18 0.21
-
 ATP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.18 Co2+ can partially replace Mg2+ in activation of ATP-diphosphate exchange Escherichia coli
6.1.1.18 Mg2+ required Escherichia coli
6.1.1.18 Mg2+ optimal MgCl2/ATP ratio is 2-5 at 2 mM ATP in aminoacylation Escherichia coli
6.1.1.18 Mn2+ can replace Mg2+ in activation of aminoacylation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.18 65400 71200 sucrose density gradient sedimentation Escherichia coli
6.1.1.18 68500
-
 electrophoresis of the native enzyme in polyacrylamide gels of various concentrations Escherichia coli
6.1.1.18 69000
-
 1 * 69000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.18 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.18
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.18 1.28
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Escherichia coli AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.18 additional information lack of tRNA-independent diphosphate exchange Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.18 monomer 1 * 69000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.18 45
-
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.18 3.3
-
 tRNAGln
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.18 6.2
-
 ATP-diphosphate exchange Escherichia coli
6.1.1.18 8.5
-
 aminoacylation Escherichia coli