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Literature summary extracted from

  • Shimizu, T.
    Bovine platelet myosin. Properties of Mg- and Ca-ATPase (1978), J. Biochem., 84, 607-616.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.8 actin
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.8 0.0001
-
 ATP Mg-ATPase, 0.6 M KCl Bos taurus
5.6.1.8 0.01
-
 ATP Ca-ATPase, 2.0 mM Ca2+ Bos taurus
5.6.1.8 0.25
-
 ATP K+EDTA-ATPase, 1 mM EDTA Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.8 ATP + H2O + myosin bound to actin filament at position n Bos taurus nucleoside triphosphates are hydrolyzed in the following order: GTP, ITP, CTP, ATP, UTP ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.8 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.8 platelet
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.8 0.0003
-
 substrate UTP, basal myosin ATPase, 0.6 M KCl, 2 mM MgCl2, pH 7 Bos taurus
5.6.1.8 0.003
-
 substrate ATP, basal myosin ATPase, 0.6 M KCl, 2 mM MgCl2, pH 7 Bos taurus
5.6.1.8 0.004
-
 substrate CTP, basal myosin ATPase, 0.6 M KCl, 2 mM MgCl2, pH 7 Bos taurus
5.6.1.8 0.005
-
 substrate ITP, basal myosin ATPase, 0.6 M KCl, 2 mM MgCl2, pH 7 Bos taurus
5.6.1.8 0.007
-
 substrate GTP, basal myosin ATPase, 0.6 M KCl, 2 mM MgCl2, pH 7 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.8 ATP + H2O + myosin bound to actin filament at position n nucleoside triphosphates are hydrolyzed in decreasing order: GTP, ITP, CTP, ATP, UTP Bos taurus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 ATP + H2O + myosin bound to actin filament at position n nucleoside triphosphates are hydrolyzed in the following order: GTP, ITP, CTP, ATP, UTP Bos taurus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 CTP + H2O + myosin bound to actin filament at position n
-
 Bos taurus CDP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 GTP + H2O + myosin bound to actin filament at position n
-
 Bos taurus GDP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 ITP + H2O + myosin bound to actin filament at position n
-
 Bos taurus IDP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 UTP + H2O + myosin bound to actin filament at position n
-
 Bos taurus UDP + phosphate + myosin bound to actin filament at position n+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.8 5
-
 platelet myosin, Mg-ATPase at 25°C, high ionic strength, 0.6 M KCl Bos taurus
5.6.1.8 9.5
-
 platelet myosin, Mg-ATPase at 25°C, high ionic strength, 0.6 M KCl Bos taurus
5.6.1.8 9.5
-
 platelet myosin, Ca-ATPase at 25°C, high ionic strength, 0.6 M KCl Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.6.1.8 5 10 Mg-ATPase, acidic maximum at pH 5.0, alkaline maximum at pH 9.5, high ionic strength, 0.6 M KCl Bos taurus
5.6.1.8 5 10 Ca-ATPase, acidic maximum at pH 5.0, alkaline maximum (higher) at pH 9.5, high ionic strength, 0.6 M KCl Bos taurus