EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.9 | Cu2+ | 50% inhibition at 1.5 mM | Lens culinaris | |
3.6.1.9 | diethyldiphosphate | inactivation follows apparent first-order kinetics, completely restored by hydroxylamine | Lens culinaris | |
3.6.1.9 | EDTA | 50% inhibition at 0.2 mM | Lens culinaris | |
3.6.1.9 | Ni2+ | 50% inhibition at 2 mM | Lens culinaris | |
3.6.1.9 | Zn2+ | 50% inhibition at 20 mM | Lens culinaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.042 | - |
NADH | - |
Lens culinaris | |
3.6.1.9 | 0.043 | - |
NADP+ | - |
Lens culinaris | |
3.6.1.9 | 0.047 | - |
NADPH | - |
Lens culinaris | |
3.6.1.9 | 0.049 | - |
NAD+ | - |
Lens culinaris | |
3.6.1.9 | 0.066 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | - |
Lens culinaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.9 | Ca2+ | activation | Lens culinaris | |
3.6.1.9 | Mg2+ | activation | Lens culinaris | |
3.6.1.9 | Mn2+ | 100% activation at 20 mM | Lens culinaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 75000 | - |
gel filtration | Lens culinaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.9 | 3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O | Lens culinaris | - |
2 ATP + 2 ADP + 2 AMP | - |
? | |
3.6.1.9 | ADP-ribose + H2O | Lens culinaris | low activity | AMP + phosphoribose | - |
? | |
3.6.1.9 | alpha-NADH + H2O | Lens culinaris | low activity | alpha-NMNH + AMP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1, P2-diphosphate + H2O | Lens culinaris | - |
AMP + AMP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1,P3-triphosphate + H2O | Lens culinaris | - |
AMP + ADP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1,P5-pentaposphate + H2O | Lens culinaris | - |
ATP + ADP + AMP + adenosine 5'-tetraphosphate | - |
? | |
3.6.1.9 | FAD + H2O | Lens culinaris | low activity | FMN + AMP | - |
? | |
3.6.1.9 | NAD+ + H2O | Lens culinaris | - |
NMN + AMP | - |
? | |
3.6.1.9 | NADH + H2O | Lens culinaris | best natural substrate, reduced form more rapidly hydrolysed than oxidized form | NMNH + AMP | - |
? | |
3.6.1.9 | NADP+ + H2O | Lens culinaris | - |
NMN + adenosine 2',5'-phosphate | - |
? | |
3.6.1.9 | NADPH + H2O | Lens culinaris | reduced form more rapidly hydrolysed than oxidized form | NMNH + adenosine 2',5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.9 | Lens culinaris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.9 | to homogeneity, chromatography techniques | Lens culinaris |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.6.1.9 | a nucleoside triphosphate + H2O = a nucleotide + diphosphate | substrates include NAD+, NADP+, FAD, CoA and also ATP and ADP | Lens culinaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.9 | seedling | - |
Lens culinaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 16.9 | - |
- |
Lens culinaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.9 | 3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O | - |
Lens culinaris | 2 ATP + 2 ADP + 2 AMP | - |
? | |
3.6.1.9 | ADP-ribose + H2O | low activity | Lens culinaris | AMP + phosphoribose | - |
? | |
3.6.1.9 | alpha-NADH + H2O | low activity | Lens culinaris | alpha-NMNH + AMP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1, P2-diphosphate + H2O | - |
Lens culinaris | AMP + AMP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1,P3-triphosphate + H2O | - |
Lens culinaris | AMP + ADP | - |
? | |
3.6.1.9 | diadenosine 5',5''-P1,P5-pentaposphate + H2O | - |
Lens culinaris | ATP + ADP + AMP + adenosine 5'-tetraphosphate | - |
? | |
3.6.1.9 | FAD + H2O | low activity | Lens culinaris | FMN + AMP | - |
? | |
3.6.1.9 | NAD+ + H2O | - |
Lens culinaris | NMN + AMP | - |
? | |
3.6.1.9 | NADH + H2O | best natural substrate, reduced form more rapidly hydrolysed than oxidized form | Lens culinaris | NMNH + AMP | - |
? | |
3.6.1.9 | NADP+ + H2O | - |
Lens culinaris | NMN + adenosine 2',5'-phosphate | - |
? | |
3.6.1.9 | NADPH + H2O | reduced form more rapidly hydrolysed than oxidized form | Lens culinaris | NMNH + adenosine 2',5'-phosphate | - |
? | |
3.6.1.9 | P1-5'-(7-methyl)-guanosine-P3-5'-guanosine triphosphate + H2O | cap analogue | Lens culinaris | 7-methylguanosine 5'-diphosphate + GMP | - |
? | |
3.6.1.9 | thymidine 5'-monophosphate p-nitrophenyl ester + H2O | faster cleavage than other substrates tested | Lens culinaris | thymidine 5'-monophosphate + p-nitrophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.9 | monomer | 1 * 75000, SDS-PAGE | Lens culinaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 10 | - |
- |
Lens culinaris |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.2 | - |
EDTA | - |
Lens culinaris | |
3.6.1.9 | 1.5 | - |
Cu2+ | - |
Lens culinaris | |
3.6.1.9 | 2 | - |
Ni2+ | - |
Lens culinaris | |
3.6.1.9 | 20 | - |
Zn2+ | - |
Lens culinaris |