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Literature summary extracted from

  • Raijman, L.; Grisolia, S.; Edelhoch, H.
    Further purification and properties of brain acyl phosphatase (1960), J. Biol. Chem., 235, 2340-2342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.7 inosinic acid brain enzyme, no effect up to 2.3 mM, 4.6 mM: 33% activation, further increase in concentration, does not produce further activation Bos taurus
3.6.1.7 papain brain enzyme, slight, 15%, activation Bos taurus

General Stability

EC Number General Stability Organism
3.6.1.7 brain enzyme, stable to proteolytic enzymes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.7 adrenaline brain enzyme, at high concentrations, 12.5 mM: 65% inhibition Bos taurus
3.6.1.7 dinitrophenol brain enzyme, 0.25 mM: 50% inhibition Bos taurus
3.6.1.7 guanidine brain enzyme, 5 M: 100% reversible inhibition, 95% of original activity is recovered upon lowering the guanidine concentration by dilution Bos taurus
3.6.1.7 additional information brain enzyme, no inhibition by caffeine, inosinic acid, 0.5% trypsin and 1% papain Bos taurus
3.6.1.7 Pepsin brain enzyme, 2%: inactivation Bos taurus
3.6.1.7 phosphate brain enzyme 20 mM: 50-60% inhibition, 40 mM: 70-80% inhibition Bos taurus
3.6.1.7 thyroxine brain enzyme, more inhibitory when preincubation is conducted at 16°C than at 25°C Bos taurus
3.6.1.7 Urea brain enzyme, 5 M: 100% reversible inhibition, 95% of original activity is recovered upon lowering the urea concentration by dilution Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.7 13200
-
 brain, ultracentrifugation Bos taurus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.1.7 Ethanol unstable to Bos taurus
3.6.1.7 Methanol unstable to Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7 brain Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.7 2638
-
 brain Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 acetyl phosphate + H2O
-
 Bos taurus acetate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Bos taurus carboxylate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Bos taurus carbamate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.7 monomer brain enzyme, 1 * 13200, ultracentrifugation Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.7 27
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.7 additional information
-
 enzyme is thermostable Bos taurus
3.6.1.7 37
-
 brain enzyme, purified and dialyzed enzyme, 20 min, 27% inactivation, undialyzed enzyme: no inactivation Bos taurus
3.6.1.7 80
-
 brain enzyme, partially purified, 15 min, pH 1: 100% activity retained, pH 7: 80-90% activity retained Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 6
-
 assay at Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.7 additional information
-
 brain enzyme, stable over a wide pH range Bos taurus
3.6.1.7 1.5 8.2 brain enzyme, 24 h: no decrease of activity Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.7 0.25
-
 dinitrophenol brain enzyme Bos taurus