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Literature summary extracted from

  • Yates, M.G.
    A non-specific adenine nucleotide deaminase from Desulfovibrio desulfuricans (1969), Biochim. Biophys. Acta, 171, 299-310.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.4.17 stable to several cycles of freezing and thawing Desulfovibrio desulfuricans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.17 2'-AMP inhibits deamination of ATP Desulfovibrio desulfuricans
3.5.4.17 3'-AMP inhibits deamination of ATP Desulfovibrio desulfuricans
3.5.4.17 adenosine inhibits deamination of ATP Desulfovibrio desulfuricans
3.5.4.17 Ag+ 0.5 mM, complete inhibition of deamination of ATP Desulfovibrio desulfuricans
3.5.4.17 beta,beta-dimethyl glutarate
-
 Desulfovibrio desulfuricans
3.5.4.17 cacodylate
-
 Desulfovibrio desulfuricans
3.5.4.17 citrate
-
 Desulfovibrio desulfuricans
3.5.4.17 Cl-
-
 Desulfovibrio desulfuricans
3.5.4.17 CN- non-competitive inhibition at low concentrations Desulfovibrio desulfuricans
3.5.4.17 Co2+
-
 Desulfovibrio desulfuricans
3.5.4.17 CuSO4
-
 Desulfovibrio desulfuricans
3.5.4.17 F- non-competitive inhibition at low concentrations Desulfovibrio desulfuricans
3.5.4.17 Fe3+
-
 Desulfovibrio desulfuricans
3.5.4.17 Hg2+ 0.5 mM, complete inhibition of deamination of ATP Desulfovibrio desulfuricans
3.5.4.17 Maleate
-
 Desulfovibrio desulfuricans
3.5.4.17 Mn2+
-
 Desulfovibrio desulfuricans
3.5.4.17 p-mercuribenzoate
-
 Desulfovibrio desulfuricans
3.5.4.17 phosphate
-
 Desulfovibrio desulfuricans
3.5.4.17 succinate
-
 Desulfovibrio desulfuricans
3.5.4.17 Urea 0.2-1 M Desulfovibrio desulfuricans
3.5.4.17 Zn2+
-
 Desulfovibrio desulfuricans

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.17 Desulfovibrio desulfuricans
-
 var. azotovorans strain Berre S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.17
-
 Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.17 26.4
-
-
 Desulfovibrio desulfuricans

Storage Stability

EC Number Storage Stability Organism
3.5.4.17 -20°C, indefinitely stable Desulfovibrio desulfuricans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.17 adenosine + H2O
-
 Desulfovibrio desulfuricans inosine + NH3
-
 ir
3.5.4.17 ADP + H2O
-
 Desulfovibrio desulfuricans IDP + NH3
-
 ir
3.5.4.17 ADP-ribose + H2O
-
 Desulfovibrio desulfuricans IDP-ribose + NH3
-
 ir
3.5.4.17 AMP + H2O
-
 Desulfovibrio desulfuricans IMP + NH3
-
 ir
3.5.4.17 ATP + H2O
-
 Desulfovibrio desulfuricans ITP + NH3
-
 ir
3.5.4.17 dADP + H2O
-
 Desulfovibrio desulfuricans dIDP + NH3
-
 ir
3.5.4.17 dAMP + H2O
-
 Desulfovibrio desulfuricans dIMP + NH3
-
 ir
3.5.4.17 dATP + H2O
-
 Desulfovibrio desulfuricans dITP + NH3
-
 ir
3.5.4.17 deoxyadenosine + H2O
-
 Desulfovibrio desulfuricans deoxyinosine + NH3
-
 ir
3.5.4.17 NAD+ + H2O
-
 Desulfovibrio desulfuricans nicotinamide-hypoxanthine dinucleotide + NH3
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.17 22
-
 stable for 3 days at room temperature Desulfovibrio desulfuricans
3.5.4.17 60
-
 5 min, complete loss of activity Desulfovibrio desulfuricans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.17 5.5 6.5 deamination of ATP Desulfovibrio desulfuricans