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Literature summary extracted from

  • Pardee, B.P.
    Hyperproduction and purification of nicotinamide deamidase, a microconstitutive enzyme of Escherichia coli (1971), J. Biol. Chem., 246, 6792-6796.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.07
-
 nicotinamide
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 30000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Escherichia coli
-
 K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.19 27.2
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.1.19 0°C, phosphate buffer, pH 7.0, weak loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Escherichia coli nicotinate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.19 37
-
 half-life: 2 h, stable in presence of 0.05 mM nicotinamide Escherichia coli
3.5.1.19 50
-
 5 min, 50% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 7.2
-
-
 Escherichia coli