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Literature summary extracted from

  • Schirmer, A.; Jendrossek, D.; Schlegel, H.G.
    Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: putification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13 (1993), Appl. Environ. Microbiol., 59, 1220-1227.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 additional information not inhibited by phenylmethylsulfonyl fluoride Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Pseudomonas fluorescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 25000
-
 gel filtration of SDS-treated enzyme Pseudomonas fluorescens
3.1.1.76 48000
-
 gel filtration of native enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas fluorescens utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas fluorescens GK13 utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas fluorescens
-
 GK13
-
3.1.1.76 Pseudomonas fluorescens GK13
-
 GK13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 purified from culture medium Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.76 culture medium
-
 Pseudomonas fluorescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 fatty acid p-nitrophenyl esters + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.1.1.76 fatty acid p-nitrophenyl esters + H2O
-
 Pseudomonas fluorescens GK13 ?
-
 ?
3.1.1.76 additional information completely inactive on poly(3hydroxybutyrate) and other short chain polyhydroxyalkanoates Pseudomonas fluorescens ?
-
 ?
3.1.1.76 additional information completely inactive on poly(3hydroxybutyrate) and other short chain polyhydroxyalkanoates Pseudomonas fluorescens GK13 ?
-
 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O acts also on copolymers, only with medium chain lenghth compounds Pseudomonas fluorescens poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O acts also on copolymers, only with medium chain lenghth compounds Pseudomonas fluorescens poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x di-3-hydroxyoctanoic acid as main product ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas fluorescens poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O acts also on copolymers, only with medium chain lenghth compounds Pseudomonas fluorescens GK13 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O acts also on copolymers, only with medium chain lenghth compounds Pseudomonas fluorescens GK13 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x di-3-hydroxyoctanoic acid as main product ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas fluorescens GK13 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 dimer gel filtration results of native and SDS-treated enzyme Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 30 32
-
 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.76 4 45
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8.5
-
-
 Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.76 6.5 9.3
-
 Pseudomonas fluorescens