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Literature summary extracted from

  • McGrath, J.W.; Wisdom, G.B.; McMullan, G.; Larkin, M.J.; Quinn, J.P.
    The purification and properties of phosphonoacetate hydrolase, a novel carbon-phosphorus bond-cleavage enzyme from Pseudomonas fluorescens 23F (1995), Eur. J. Biochem., 234, 225-230.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.11.1.2 analysis activity stain for detection of carbon-phosphorus cleavage by phosphonoacetate hydrolase in PAGE gels Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.11.1.2 1,10-phenanthroline 1 mM, complete inhibition Pseudomonas fluorescens
3.11.1.2 3-phosphonopropionate
-
 Pseudomonas fluorescens
3.11.1.2 EDTA 5 mM, complete inhibition Pseudomonas fluorescens
3.11.1.2 Phosphonoacetate substrate inhibition at 25 mM and above Pseudomonas fluorescens
3.11.1.2 Phosphonoformate
-
 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.11.1.2 1.25
-
 Phosphonoacetate
-
 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.11.1.2 Co2+ enhances activity Pseudomonas fluorescens
3.11.1.2 Mn2+ enhances activity Pseudomonas fluorescens
3.11.1.2 Zn2+ enhances activity, best activation at 1 mM Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.11.1.2 38000
-
 2 * 38000, SDS-PAGE Pseudomonas fluorescens
3.11.1.2 80000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.11.1.2 Pseudomonas fluorescens
-
-
-
3.11.1.2 Pseudomonas fluorescens 23F
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.11.1.2
-
 Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.11.1.2 culture condition:phosphonoacetate-grown cell phosphonoacetate as the sole carbon and phosphorus source Pseudomonas fluorescens
-

Storage Stability

EC Number Storage Stability Organism
3.11.1.2 -20°C, 35% loss of activity after 31 days, 45% loss of activity after 34 days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.11.1.2 Phosphonoacetate + H2O
-
 Pseudomonas fluorescens acetate + phosphate
-
 ?
3.11.1.2 Phosphonoacetate + H2O
-
 Pseudomonas fluorescens 23F acetate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.11.1.2 dimer 2 * 38000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.11.1.2 37
-
-
 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.11.1.2 20 45 20°C: about 40% of maximal activity, 45°C: about 50% of maximal activity Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.11.1.2 40
-
 30 min, rapid loss of activity Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.11.1.2 3
-
 Phosphonoacetate
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.11.1.2 7.8
-
-
 Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.11.1.2 7 9.6 pH 7.0: about 65% of maximal activity, pH 9.6: about 75% of maximal activity Pseudomonas fluorescens