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Literature summary extracted from

  • Frassetto, S.S.; Dias, R.D.; Sarkis, J.J.
    Characterization of an ATP diphosphohydrolase activity (apyrase EC 3.6.1.5) in rat blood platelets (1993), Mol. Cell. Biochem., 129, 47-55.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 additional information no inhibition by lanthanum; no inhibition by NEM; no inhibition by oligomycin; no inhibition by orthovanadate; no inhibition by ouabain Rattus norvegicus
3.6.1.5 NaN3 above 5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.024
-
 ATP CaATP2- Rattus norvegicus
3.6.1.5 0.031
-
 ADP CaADP- Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane ectonucleotidase/ectoenzyme Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ required for activity Rattus norvegicus
3.6.1.5 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Rattus norvegicus
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Rattus norvegicus
-
 AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 blood platelet
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 0.004
-
 ADP, presence of Ca2+ Rattus norvegicus
3.6.1.5 0.007
-
 ATP, presence of Ca2+ Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Rattus norvegicus AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O
-
 Rattus norvegicus CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O
-
 Rattus norvegicus CMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O
-
 Rattus norvegicus GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Rattus norvegicus GMP + 2 phosphate
-
 ?
3.6.1.5 IDP + H2O
-
 Rattus norvegicus IMP + phosphate
-
 ?
3.6.1.5 ITP + 2 H2O
-
 Rattus norvegicus IMP + 2 phosphate
-
 ?
3.6.1.5 UDP + H2O
-
 Rattus norvegicus UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O best substrate Rattus norvegicus UMP + 2 phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.5 8
-
 Rattus norvegicus