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Literature summary extracted from

  • Heine, P.; Braun, N.; Heilbronn, A.; Zimmermann, H.
    Functional characterization of rat ecto-ATPase and ecto-ATP diphosphohydrolase after heterologous expression in CHO cells (1999), Eur. J. Biochem., 262, 102-107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 expression in cells of chinese hamster ovaries Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 EDTA 1 mM Rattus norvegicus
3.6.1.5 Evans blue complete inhibition at 0.1 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane ectonucleotidase/ectoenzyme Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ either Mg2+ or Ca2+ required for activity Rattus norvegicus
3.6.1.5 Mg2+ either Mg2+ or Ca2+ required for activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 70000 80000 SDS-PAGE, immunoblotting Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Rattus norvegicus
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Rattus norvegicus
-
 AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.5 ADP + H2O 87% relative activity compared to ATP Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Rattus norvegicus AMP + 2 phosphate
-
 ?
3.6.1.5 ATP + 2 H2O 100% relative activity Rattus norvegicus AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O lowest relative activity with 61% Rattus norvegicus CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O
-
 Rattus norvegicus CMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O
-
 Rattus norvegicus GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Rattus norvegicus GMP + 2 phosphate
-
 ?
3.6.1.5 IDP + H2O
-
 Rattus norvegicus IMP + phosphate
-
 ?
3.6.1.5 ITP + 2 H2O
-
 Rattus norvegicus IMP + 2 phosphate
-
 ?
3.6.1.5 additional information neglible hydrolysis of AMP Rattus norvegicus ?
-
 ?
3.6.1.5 UDP + H2O
-
 Rattus norvegicus UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Rattus norvegicus UMP + 2 phosphate
-
 ?