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Literature summary extracted from
- Cloning and expression in Escherichia coli of the glutamate racemase gene from Pediococcus pentosaceus (1986), Agric. Biol. Chem., 50, 2823-2830.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | expression in Escherichia coli | Pediococcus pentosaceus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.1.1.3 | irreversible loss of activity on storage at DL-Glu and a reducing thiol such as 2-mercaptoethanol. Inactivation may be due to air oxidation of a free thiol residue | Pediococcus pentosaceus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.3 | 2-Nitro-5-thiocyanobenzoate | - |
Pediococcus pentosaceus |  |
| 5.1.1.3 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Pediococcus pentosaceus | |
| 5.1.1.3 | hydroxylamine | no inactivation | Pediococcus pentosaceus | |
| 5.1.1.3 | additional information | no inactivation by carbonyl reagents such as hydroxylamine and NaBH4 | Pediococcus pentosaceus | |
| 5.1.1.3 | p-chloromercuribenzoate | - |
Pediococcus pentosaceus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 15 | - |
D-Glu | - |
Pediococcus pentosaceus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 40000 | - |
HPLC gel filtration | Pediococcus pentosaceus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.3 | Pediococcus pentosaceus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | - |
Pediococcus pentosaceus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 26 | - |
- |
Pediococcus pentosaceus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.3 | D-Glu | - |
Pediococcus pentosaceus | L-Glu | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.3 | monomer | 1 * 40000, SDS-PAGE | Pediococcus pentosaceus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 40 | - |
- |
Pediococcus pentosaceus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 8 | - |
- |
Pediococcus pentosaceus |
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