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Literature summary extracted from

  • Chou, C.S.; Rodwell, V.W.
    Metabolism of basic amino acids in Pseudomonas putida gamma-guanidinobutyrate amidinohydrolase (1972), J. Biol. Chem., 247, 4486-4490.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 30
-
 4-Guanidinobutyrate
-
 Gallus sp.
3.5.3.7 32
-
 4-Guanidinobutyrate at pH 10 Pseudomonas putida
3.5.3.7 41
-
 4-Guanidinobutyrate at pH 9 Pseudomonas putida
3.5.3.7 46
-
 4-Guanidinobutyrate at pH 11 Pseudomonas putida
3.5.3.7 163
-
 6-Guanidinocaproate at pH 10 Pseudomonas putida
3.5.3.7 206
-
 5-Guanidinovalerate at pH 10 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.7 mitochondrion
-
 Helix pomatia 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Mn2+
-
 Pseudomonas fluorescens
3.5.3.7 Mn2+
-
 Streptomyces griseus
3.5.3.7 Mn2+
-
 Penicillium roqueforti
3.5.3.7 Mn2+
-
 Iguania
3.5.3.7 Mn2+
-
 Raja clavata
3.5.3.7 Mn2+ requires Mn2+, activation and reactivation of EDTA treated enzyme by MnSO4 Pseudomonas putida
3.5.3.7 Mn2+ activated by Helix pomatia
3.5.3.7 Mn2+ activated by Gallus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 178000
-
 gel filtration Pseudomonas putida
3.5.3.7 190000
-
 sucrose density gradient centrifugation Pseudomonas putida
3.5.3.7 252000
-
-
 Iguania

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas putida
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Streptomyces griseus
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Helix pomatia
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Penicillium roqueforti
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Gallus sp.
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Iguania
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Raja clavata
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Gallus sp.
-
 chicken
-
3.5.3.7 Helix pomatia
-
 snail
-
3.5.3.7 Iguania
-
-
-
3.5.3.7 Penicillium roqueforti
-
-
-
3.5.3.7 Pseudomonas fluorescens
-
-
-
3.5.3.7 Pseudomonas putida
-
 P2 (ATCC 25571)
-
3.5.3.7 Raja clavata
-
 ray
-
3.5.3.7 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Pseudomonas fluorescens
3.5.3.7
-
 Pseudomonas putida
3.5.3.7
-
 Helix pomatia
3.5.3.7
-
 Gallus sp.
3.5.3.7
-
 Iguania
3.5.3.7
-
 Raja clavata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.7 hepatopancreas
-
 Helix pomatia
-
3.5.3.7 kidney
-
 Gallus sp.
-
3.5.3.7 liver
-
 Gallus sp.
-
3.5.3.7 liver
-
 Iguania
-
3.5.3.7 liver
-
 Raja clavata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 64
-
 EDTA treated, 0.2 mM Co(NO3)2 Pseudomonas putida
3.5.3.7 69
-
 EDTA treated Pseudomonas putida
3.5.3.7 79
-
 EDTA treated, 0.2 mM MnSO4 Pseudomonas putida
3.5.3.7 158
-
 EDTA treated, 0.02 mM MnSO4 Pseudomonas putida
3.5.3.7 198
-
 EDTA treated, 0.04 mM MnSO4 Pseudomonas putida
3.5.3.7 209
-
 EDTA treated, 0.12 mM MnSO4 Pseudomonas putida
3.5.3.7 290
-
 EDTA treated, 0.2 mM MnSO4 Pseudomonas putida
3.5.3.7 303
-
 purified enzyme Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.3.7 -20°C stable to storage for at least 1 month Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas putida 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Streptomyces griseus 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Helix pomatia 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Penicillium roqueforti 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Gallus sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Iguania 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Raja clavata 4-Aminobutyrate + urea
-
 ?
3.5.3.7 5-guanidinovalerate + H2O
-
 Pseudomonas fluorescens 5-aminopentanoate + urea
-
 ?
3.5.3.7 5-guanidinovalerate + H2O
-
 Pseudomonas putida 5-aminopentanoate + urea
-
 ?
3.5.3.7 6-guanidinocaproate + H2O
-
 Pseudomonas fluorescens 6-aminohexanoate + urea
-
 ?
3.5.3.7 6-guanidinocaproate + H2O
-
 Pseudomonas putida 6-aminohexanoate + urea
-
 ?
3.5.3.7 additional information
-
 Streptomyces griseus ?
-
 ?
3.5.3.7 additional information guanidinoacetate, beta-guanidinopropionate, L-alpha-amino-gamma-guanidinobutyrate, L-arginine, D-arginine, L-homoarginine and agmatine are no substrates Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.7 40
-
-
 Penicillium roqueforti
3.5.3.7 50
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 7.5 8.5
-
 Penicillium roqueforti
3.5.3.7 9.5
-
-
 Pseudomonas fluorescens
3.5.3.7 9.5
-
-
 Pseudomonas putida
3.5.3.7 9.5
-
-
 Streptomyces griseus
3.5.3.7 9.5
-
-
 Helix pomatia
3.5.3.7 9.5
-
-
 Penicillium roqueforti
3.5.3.7 9.5
-
-
 Gallus sp.
3.5.3.7 9.5
-
-
 Iguania
3.5.3.7 9.5
-
-
 Raja clavata
3.5.3.7 9.9
-
-
 Pseudomonas putida
3.5.3.7 10
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.7 7 11
-
 Pseudomonas putida