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Literature summary extracted from

  • Prasad, G.L.; Adiga, P.R.
    Purification and characterization of putrescine synthase from cucumber seedlings. A multifunctional enzyme involved in putrescine biosynthesis (1986), J. Biosci., 10, 373-391.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.3.12 highly unstable Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.6 15000
-
 x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol Cucumis sativus
2.1.3.6 18000
-
 x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol Cucumis sativus
2.1.3.6 44000
-
 x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol Cucumis sativus
2.1.3.6 150000
-
 x * 150000, SDS-PAGE, nonreducing Cucumis sativus
3.5.3.12 15000
-
 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE Cucumis sativus
3.5.3.12 44000
-
 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE Cucumis sativus
3.5.3.12 48000
-
 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE Cucumis sativus
3.5.3.12 66000
-
 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE Cucumis sativus
3.5.3.12 150000
-
 gel filtration Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.12 agmatine + H2O Lathyrus sativus
-
 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Cucumis sativus
-
 N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.6 Cucumis sativus
-
-
-
3.5.3.12 Cucumis sativus
-
 cucumber
-
3.5.3.12 Lathyrus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12
-
 Lathyrus sativus
3.5.3.12
-
 Cucumis sativus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.6 carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine multifunctional enzyme that catalyzes the reactions of EC 2.1.3.3 ornithine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively Cucumis sativus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.6 seedling
-
 Cucumis sativus
-
3.5.3.12 cotyledon
-
 Cucumis sativus
-
3.5.3.12 seedling
-
 Lathyrus sativus
-
3.5.3.12 seedling
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.12 2.64
-
-
 Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + ADP + phosphate
-
 Cucumis sativus ATP + putrescine + NH3 + CO2
-
 ?
3.5.3.12 agmatine + H2O
-
 Lathyrus sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Cucumis sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase Lathyrus sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase Cucumis sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + ornithine + H2O + phosphate
-
 Lathyrus sativus putrescine + citrulline + NH3 + phosphate
-
 ?
3.5.3.12 agmatine + ornithine + H2O + phosphate
-
 Cucumis sativus putrescine + citrulline + NH3 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.6 ? x * 150000, SDS-PAGE, nonreducing Cucumis sativus
2.1.3.6 ? x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol Cucumis sativus
3.5.3.12 oligomer 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
3.5.3.12 putrescine synthase
-
 Lathyrus sativus
3.5.3.12 putrescine synthase
-
 Cucumis sativus