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Literature summary extracted from

  • Williamson, J.M.; Meister, A.
    Effect of sulfhydryl group modification on the activities of 5-oxo-L-prolinase (1982), J. Biol. Chem., 257, 9161-9172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.9 DTNB could be reactivated with dithiothreitol Rattus norvegicus
3.5.2.9 N-ethylmaleimide
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.9 0.008
-
 5-oxo-L-proline
-
 Rattus norvegicus
3.5.2.9 0.009
-
 5-oxo-L-proline treated with N-ethylmaleimide Rattus norvegicus
3.5.2.9 0.13
-
 ITP measured in absence of 5-oxoproline Rattus norvegicus
3.5.2.9 0.17
-
 ATP
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.9 Ca2+ promotes activity significantly Rattus norvegicus
3.5.2.9 Co2+ promotes activity significantly Rattus norvegicus
3.5.2.9 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.9 115000
-
 4 * 115000, SDS-PAGE Rattus norvegicus
3.5.2.9 142000
-
 2 * 142000, SDS-PAGE Rattus norvegicus
3.5.2.9 325000
-
 gel filtration Rattus norvegicus
3.5.2.9 420000
-
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.9 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.9 7
-
-
 Rattus norvegicus
3.5.2.9 87
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 2-imidazolidone-4-carboxylate + ATP + H2O promotes enzymatic hydrolysis of ATP but is not itself hydrolyzed Rattus norvegicus 2-imidazolidone + ADP + phosphate
-
 ?
3.5.2.9 5'-p-fluorosulfonylbenzoyladenosine + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 5'-p-fluorosulfonylbenzoylinosine + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 ATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 GTP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 ITP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 UTP + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.9 dimer 2 * 142000, SDS-PAGE Rattus norvegicus
3.5.2.9 tetramer 4 * 115000, SDS-PAGE Rattus norvegicus