Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Serrano, A.E.; Nagayama, F.
    Purification and characterization of liver arylformamidase in rainbow trout and cattle (1991), Comp. Biochem. Physiol. B, 98, 381-387.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.9 0.28
-
 N-formyl-L-kynurenine
-
 Oncorhynchus mykiss
3.5.1.9 0.45
-
 N-formyl-DL-kynurenine
-
 Oncorhynchus mykiss
3.5.1.9 0.78
-
 N-formyl-L-kynurenine
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.9 31700
-
 gel filtration Oncorhynchus mykiss
3.5.1.9 42600
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O Bos taurus involved in the pathway of tryptophan degradation L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-L-kynurenine + H2O Oncorhynchus mykiss involved in the pathway of tryptophan degradation L-kynurenine + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.9 Bos taurus
-
-
-
3.5.1.9 Oncorhynchus mykiss
-
 rainbow trout
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.9 to homogeneity, chromatography techniques Bos taurus
3.5.1.9 to homogeneity, chromatography techniques Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.9 liver
-
 Bos taurus
-
3.5.1.9 liver
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.9 425
-
-
 Bos taurus
3.5.1.9 1304
-
-
 Oncorhynchus mykiss

Storage Stability

EC Number Storage Stability Organism
3.5.1.9 4°C, 60% of activity recovered after seven months Bos taurus
3.5.1.9 4°C, fully active within seven months Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.9 N-formyl-DL-kynurenine + H2O mixture of equal amounts of D- and L-kynurenine Bos taurus L-kynurenine + formate + N-formyl-D-kynurenine N-formyl-D-kynurenine might inhibit activity ?
3.5.1.9 N-formyl-DL-kynurenine + H2O mixture of equal amounts of D- and L-kynurenine Oncorhynchus mykiss L-kynurenine + formate + N-formyl-D-kynurenine N-formyl-D-kynurenine might inhibit activity ?
3.5.1.9 N-formyl-L-kynurenine + H2O involved in the pathway of tryptophan degradation Bos taurus L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-L-kynurenine + H2O involved in the pathway of tryptophan degradation Oncorhynchus mykiss L-kynurenine + formate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.9 40
-
-
 Bos taurus
3.5.1.9 40
-
-
 Oncorhynchus mykiss

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.9 60
-
 80% remaining activity after 30 min Bos taurus
3.5.1.9 60
-
 16% remaining activity after 30 min Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.9 7.7
-
-
 Oncorhynchus mykiss
3.5.1.9 8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.9 6.5 7.5 high activity within Bos taurus
3.5.1.9 6.5 11 high activity within Oncorhynchus mykiss

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.9 3 4 no activity Oncorhynchus mykiss
3.5.1.9 3 4 remaining activity: 20-50% Bos taurus