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Literature summary extracted from

  • De Vries, R.P.; Michelsen, B.; Poulsen, C.H.; Kroon, P.A.; van den Heuvel, R.H.; Faulds, C.B.; Williamson, G.; van den Hombergh, J.P.; Visser, J.
    The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides (1997), Appl. Environ. Microbiol., 63, 4638-4644.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene cinI, coding for cinnamoyl esterase Butyrivibrio fibrisolvens
3.1.1.73 PCR cloning and sequence analysis, gene faeA encoding ferulic acid esterase cloned, the gene product FAEA overexpressed in wild-type Aspergillus tubingensis and protease-deficient Aspergillus niger mutant, gene product is identical with Aspergillus niger FAE-III Aspergillus niger
3.1.1.73 PCR cloning and sequence analysis, gene faeA encoding ferulic acid esterase cloned, the gene product FAEA overexpressed in wild-type Aspergillus tubingensis and protease-deficient Aspergillus niger mutant, gene product is identical with Aspergillus niger FAE-III Aspergillus tubingensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.72
-
 methyl ferulate FAE-III Aspergillus niger
3.1.1.73 0.76
-
 methyl ferulate recombinant FAEA Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 29740
-
 FAEA, mass spectrometry Aspergillus tubingensis
3.1.1.73 29740
-
 FAE-III, mass spectrometry Aspergillus niger
3.1.1.73 36000
-
-
 Aspergillus tubingensis
3.1.1.73 36000
-
 FAE-III Aspergillus niger
3.1.1.73 36000
-
 FAEA Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 arabinoxylans + H2O Aspergillus niger
-
 ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylans + H2O Aspergillus tubingensis
-
 ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylans + H2O Aspergillus niger N402
-
 ferulic acid + ?
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Butyrivibrio fibrisolvens
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus tubingensis
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger N402
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 sugar beet pectin + H2O Aspergillus tubingensis
-
 ferulic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger
-
 and strain NW219
-
3.1.1.73 Aspergillus niger N402
-
 and strain NW219
-
3.1.1.73 Aspergillus tubingensis
-
-
-
3.1.1.73 Butyrivibrio fibrisolvens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus niger
3.1.1.73 recombinant gene product Aspergillus tubingensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 arabinoxylans + H2O
-
 Aspergillus niger ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylans + H2O
-
 Aspergillus tubingensis ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylans + H2O
-
 Aspergillus niger N402 ferulic acid + ?
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus tubingensis ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus niger N402 ferulic acid + methanol
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Butyrivibrio fibrisolvens ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus tubingensis ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger N402 ferulate + polysaccharide
-
 ?
3.1.1.73 sugar beet pectin + H2O
-
 Aspergillus tubingensis ferulic acid + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55 60 FAE-III Aspergillus niger
3.1.1.73 60
-
-
 Aspergillus niger
3.1.1.73 60
-
-
 Aspergillus tubingensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Aspergillus niger
3.1.1.73 5
-
-
 Aspergillus tubingensis