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Literature summary extracted from

  • Yokoigawa, K.; Kawai, H.; Endo, K.; Lim, Y.H.; Esaki, N.; Soda, K.
    Thermolabile alanine racemase from a psychrotroph, Pseudomonas fluorescens: purification and properties (1993), Biosci. Biotechnol. Biochem., 57, 93-97.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 12.8
-
 D-Ala
-
 Pseudomonas fluorescens
5.1.1.1 18.9
-
 L-Ala
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 38000
-
 2 * 38000, SDS-PAGE Pseudomonas fluorescens
5.1.1.1 76000
-
 gel filtration in presence or absence of 2-mercaptoethanol Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 587
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
5.1.1.1 -20°C, stable for 1 month Pseudomonas fluorescens
5.1.1.1 4°C, stable for 1 week Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala
-
 Pseudomonas fluorescens D-Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 38000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 30
-
-
 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.1.1
-
 40 0°C: about 25% of maximal activity, 40°C: about 45% of maximal activity Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.1 20
-
 1 h, stable Pseudomonas fluorescens
5.1.1.1 30
-
 over 30°C, 1 h, quick loss of activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 8.3
-
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate bound per mol of enzyme dimer Pseudomonas fluorescens