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Literature summary extracted from

  • Vicente, O.; Filipowicz, W.
    Purification of RNA 3'-terminal phosphate cyclase from HeLa cells (1988), Eur. J. Biochem., 17, 431-439.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.5.1.4 not affected by several cycles of freezing and thawing Homo sapiens
6.5.1.4 resistant to protease digestion Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.4 iodoacetamide
-
 Homo sapiens
6.5.1.4 NEM
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.4 0.006
-
 ATP
-
 Homo sapiens
6.5.1.4 0.006
-
 ATPgammaS ATP Homo sapiens
6.5.1.4 0.2
-
 GTP
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.4 Ca2+ only 5% of the activation by Mg2+ Homo sapiens
6.5.1.4 K+ optimal concentration: 150-200 mM Homo sapiens
6.5.1.4 Mg2+ required Homo sapiens
6.5.1.4 Mg2+ maximal activity at 4-5 mM Homo sapiens
6.5.1.4 Mn2+ only 5% of the activation by Mg2+ Homo sapiens
6.5.1.4 Na+ optimal concentration: 150-200 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.4 38000 40000 glycerol density gradient sedimentation, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.4
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.4 ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside formation of a covalent cyclase-AMP intermediate Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.4 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.4 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.5.1.4 -70°C, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.4 ATP + RNA 3'-terminal-phosphate no activity with dATP Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATPgammaS + RNA 3'-terminal-phosphate
-
 Homo sapiens AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 CTP + RNA 3'-terminal-phosphate
-
 Homo sapiens CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 GTP + RNA 3'-terminal-phosphate
-
 Homo sapiens GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 UTP + RNA 3'-terminal-phosphate
-
 Homo sapiens UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.4 monomer 1 * 38000-40000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.4 30
-
 similar rates of reaction at 25°C, 30°C, and 37°C Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.4
-
 37 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.4 42
-
 5 min, 55% loss of activity Homo sapiens
6.5.1.4 50
-
 5 min, 95% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.4 8 9
-
 Homo sapiens