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Literature summary extracted from

  • Schiele, U.; Lynen, F.
    3-Methylcrotonyl-CoA carboxylase from Achromobacter (1981), Methods Enzymol., 71, 781-791.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.4 iodoacetamide 3-methylcrotonoyl-CoA protects against inactivation Achromobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.4 0.012
-
 3-methylcrotonoyl-CoA
-
 Achromobacter sp.
6.4.1.4 0.083
-
 ATP
-
 Achromobacter sp.
6.4.1.4 2.17
-
 HCO3-
-
 Achromobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.4 Mg2+ Mg2+ or Mn2+ required Achromobacter sp.
6.4.1.4 Mn2+ Mn2+ or Mg2+ required Achromobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.4 78000
-
 4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B Achromobacter sp.
6.4.1.4 96000
-
 4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B Achromobacter sp.
6.4.1.4 700000 760000
-
 Achromobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Achromobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.4
-
 Achromobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.4 9.36
-
-
 Achromobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonoyl-CoA + HCO3- r Achromobacter sp. ADP + phosphate + 3-methylglutaconyl-CoA
-
 ?
6.4.1.4 ATP + 3-methylcrotonoyl-CoA + HCO3- the forward reaction is about 10times faster than the reverse reaction Achromobacter sp. ADP + phosphate + 3-methylglutaconyl-CoA
-
 ?
6.4.1.4 additional information catalyzes ATP/ADP exchange reaction Achromobacter sp. ?
-
 ?
6.4.1.4 additional information catalyzes ATP/diphosphate exchange reaction Achromobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.4 octamer 4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B Achromobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.4 7.9 8.3
-
 Achromobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.4 biotin enzyme contains biotin Achromobacter sp.
6.4.1.4 biotin contains 4 molecules of biotin per enzyme complex Achromobacter sp.