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Literature summary extracted from

  • Tani, M.; Okino, N.; Mitsutake, S.; Tanigawa, T.; Izu, H.; Ito, M.
    Purification and characterization of a neutral ceramidase from mouse liver (2000), J. Biol. Chem., 275, 3462-3468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 sodium cholate
-
 Mus musculus
3.5.1.23 sodium taurodeoxycholate
-
 Mus musculus
3.5.1.23 Triton X-100
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+
-
 Mus musculus
3.5.1.23 Hg2+
-
 Mus musculus
3.5.1.23 N-oleoylethanolamine
-
 Mus musculus
3.5.1.23 sphinganine
-
 Mus musculus
3.5.1.23 sphingenine
-
 Mus musculus
3.5.1.23 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.0223
-
 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide
-
 Mus musculus
3.5.1.23 0.0724
-
 C16-14C-ceramide
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 94000
-
 x * 94000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 151182fold Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 14.45
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.5.1.23 -80°C, 2 months, no loss of activity Mus musculus
3.5.1.23 37°C, 24 h, no loss of acitivity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-N-dodecanoylsphingosine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-N-hexanoylsphingosine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 C16-14C-ceramide + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 N-palmitoylphytosphingosine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 N-palmitoylsphinganine + H2O
-
 Mus musculus palmitic acid + sphinganine
-
 ?
3.5.1.23 N-palmitoylsphingosine + H2O
-
 Mus musculus palmitic acid + sphingosine
-
 ?
3.5.1.23 N-stearoylphytosphingosine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.23 N-stearoylsphinganine + H2O
-
 Mus musculus stearic acid + sphinganine
-
 ?
3.5.1.23 N-stearoylsphingosine + H2O
-
 Mus musculus stearic acid + sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 94000, SDS-PAGE Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.23 60
-
 70% loss of activity after 1 h at 60°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 7.5
-
-
 Mus musculus