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Literature summary extracted from

  • Imai, T.
    Properties of allosteric nicotinamide mononucleotide glycohydrolase from Azotobacter vinelandii: Activation and inhibition (1987), J. Biochem., 101, 163-173.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.14 guanosine 3'-diphosphate-5'-triphosphate guanine nucleotides, guanosinetriphosphate and polyphosphate derivatives, effective activation Azotobacter vinelandii
3.2.2.14 RNA
-
 Azotobacter vinelandii
3.2.2.14 SO42- 10 mM relative activity 103%, 100% activity with nicotinamide as substrate Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.14 5-phosphoribose 1-diphosphate PRPP, inorganic polyphosphates, affinity of 5-phosphoribose 1-pyrophosphate for the enzyme is dependent on the activator concentration , inhibition mechanism with 5-phosphoribose 1-pyrophosphate is competitive with GTP Azotobacter vinelandii
3.2.2.14 7-methylguanosine-5'-triphosphate
-
 Azotobacter vinelandii
3.2.2.14 ATP
-
 Azotobacter vinelandii
3.2.2.14 CTP
-
 Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.14 0.0087
-
 guanosine 3'-diphosphate 5'-triphosphate
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.14 110000
-
 1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP Azotobacter vinelandii
3.2.2.14 210000
-
 gel filtration Azotobacter vinelandii
3.2.2.14 240000
-
-
 Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.14 nicotinamide mononucleotide + H2O Azotobacter vinelandii biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.14 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.14
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.14 guanosine 3'-diphosphate 5'-triphosphate + H2O
-
 Azotobacter vinelandii ?
-
 ?
3.2.2.14 nicotinamide mononucleotide + H2O biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 Azotobacter vinelandii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.14 dimer 1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP Azotobacter vinelandii