Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rombouts, F.M.; Voragen, A.G.J.; Searle-van Leeuwen, M.F.; Geraeds, C.C.J.M.; Schols, H.A.; Pilnik, W.
    The arabinanases of Aspergillus niger - purification and characterization of two alpha-L-arabinofuranosidases and an endo-1,5-alpha-L-arabinanase (1988), Carbohydr. Polym., 9, 25-47.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 additional information
-
 additional information
-
 Aspergillus niger
3.2.1.99 4.2
-
 1,5-alpha-L-arabinan
-
 Aspergillus niger
3.2.1.99 9.3
-
 L-arabinan apple juice ultrafiltration retentate, UFR, arabinan, linearised Aspergillus niger
3.2.1.99 26
-
 L-arabinan apple juice ultrafiltration retentate, UFR, arabinan Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 35000
-
 SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.99 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 0.36
-
 apple juice ultrafiltration retentate, UFR, arabinan Aspergillus niger
3.2.1.99 1.2
-
 best substrate: 1,5-alpha-L-arabinan Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.2.1.99 -20°C, 10 mM sodium acetate or piperazine buffer, pH 5.0 dispensed in small tubes Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O poor substrate Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O poor substrate Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 galactan + H2O potato galactan: poor substrate, citrus galactan: very poor substrate Aspergillus niger ?
-
 ?
3.2.1.99 L-arabinan + H2O beet and apple juice ultrafiltration retentate, UFR, arabinan Aspergillus niger L-arabino-oligosaccharides end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?
3.2.1.99 additional information substrate specificity, overview Aspergillus niger ?
-
 ?
3.2.1.99 additional information inactive towards synthetic arabinosides, p-nitrophenyl-alpha-L-arabinoside, arabinose disaccharide, arabinogalactan, stractan and xylan from oat spelts Aspergillus niger ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 50
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 50
-
 up to, full retention of activity, sharp drop in activity beyond 50°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 5
-
-
 Aspergillus niger