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Literature summary extracted from

  • Widmer, F.; Leuba, J.L.
    beta-Galactosidase from Aspergillus niger. Separation and characterization of three multiple forms (1979), Eur. J. Biochem., 100, 559-567.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.35
-
 o-nitrophenyl-beta-D-galactoside enzyme form 1 Aspergillus niger
3.2.1.23 2.36
-
 o-nitrophenyl-beta-D-galactoside enzyme form 2 Aspergillus niger
3.2.1.23 2.38
-
 o-nitrophenyl-beta-D-galactoside enzyme form 3 Aspergillus niger
3.2.1.23 85
-
 lactose enzyme form 1 Aspergillus niger
3.2.1.23 105
-
 lactose enzyme form 2 Aspergillus niger
3.2.1.23 125
-
 lactose enzyme form 3 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 124000
-
 enzyme form 1, gel filtration Aspergillus niger
3.2.1.23 150000
-
 enzyme form 2, gel filtration Aspergillus niger
3.2.1.23 173000
-
 enzyme form 3, gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein enzyme form 1 contains 13.5% w/w neutral carbohydrate, enzyme form 2 contains 19.2% w/w neutral carbohydrate, enzyme form 3 contains 28.5% w/w neutral carbohydrate Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 3 enzyme forms Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 23.1
-
 enzyme form 1 Aspergillus niger
3.2.1.23 77.5
-
 enzyme form 2 Aspergillus niger
3.2.1.23 82.4
-
 enzyme form 3 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Aspergillus niger D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-galactose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3
-
 enzyme form 1, 2 and 3, hydrolysis of lactose Aspergillus niger
3.2.1.23 3.2
-
 enzyme form 3, hydrolysis of o-nitrophenyl-beta-D-galactoside Aspergillus niger