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Literature summary extracted from

  • Zapater, I.G.; Ullah, A.H.J.; Wodzinsky, R.J.
    Extracellular alpha-galactosidase (e.c. 3.2.1.22) from Aspergillus ficuum NRRL 3135 purification and characterization (1990), Prep. Biochem., 20, 263-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+
-
 Aspergillus ficuum
3.2.1.22 Cu2+
-
 Aspergillus ficuum
3.2.1.22 D-galactose
-
 Aspergillus ficuum
3.2.1.22 D-glucose
-
 Aspergillus ficuum
3.2.1.22 D-mannose
-
 Aspergillus ficuum
3.2.1.22 fructose
-
 Aspergillus ficuum
3.2.1.22 Hg2+
-
 Aspergillus ficuum
3.2.1.22 Zn2+
-
 Aspergillus ficuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.72
-
 3-nitrophenyl alpha-D-galactopyranoside
-
 Aspergillus ficuum
3.2.1.22 0.84
-
 2-nitrophenyl alpha-D-galactopyranoside
-
 Aspergillus ficuum
3.2.1.22 1.46
-
 4-nitrophenyl alpha-D-galactopyranoside
-
 Aspergillus ficuum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 extracellular
-
 Aspergillus ficuum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 70700
-
 gel filtration Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Aspergillus ficuum
-
 NRRL 3135
-
3.2.1.22 Aspergillus ficuum NRRL 3135
-
 NRRL 3135
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein
-
 Aspergillus ficuum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Aspergillus ficuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 2-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum 2-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 2-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum NRRL 3135 2-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 3-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum 3-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 3-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum NRRL 3135 3-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Aspergillus ficuum NRRL 3135 4-nitrophenol + alpha-D-galactopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 60
-
-
 Aspergillus ficuum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 65
-
 10 min, 33% of activity left Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 6
-
-
 Aspergillus ficuum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 2.3
-
 12 h, 62% of activity left Aspergillus ficuum