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Literature summary extracted from
- Purification and properties of fructose-1,6-bisphosphatase of Bacillus subtilis (1979), J. Biol. Chem., 254, 5340-5349.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | NH4+ | activates | Bacillus subtilis |  |
| 3.1.3.11 | phosphoenolpyruvate | activates | Bacillus subtilis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | ADP | - |
Bacillus subtilis | |
| 3.1.3.11 | AMP | - |
Bacillus subtilis | |
| 3.1.3.11 | ATP | - |
Bacillus subtilis | |
| 3.1.3.11 | CDP | - |
Bacillus subtilis | |
| 3.1.3.11 | CTP | - |
Bacillus subtilis | |
| 3.1.3.11 | dAMP | - |
Bacillus subtilis | |
| 3.1.3.11 | denatured DNA | - |
Bacillus subtilis | |
| 3.1.3.11 | diphosphate | - |
Bacillus subtilis | |
| 3.1.3.11 | EDTA | Na2EDTA | Bacillus subtilis | |
| 3.1.3.11 | FAD | - |
Bacillus subtilis | |
| 3.1.3.11 | GDP | - |
Bacillus subtilis | |
| 3.1.3.11 | GTP | - |
Bacillus subtilis | |
| 3.1.3.11 | NAD+ | - |
Bacillus subtilis | |
| 3.1.3.11 | phosphate | - |
Bacillus subtilis | |
| 3.1.3.11 | RNA | - |
Bacillus subtilis | |
| 3.1.3.11 | UDP | - |
Bacillus subtilis | |
| 3.1.3.11 | UTP | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.11 | 0.006 | - |
fructose 1,6-diphosphate | without phosphoenolpyruvate | Bacillus subtilis | |
| 3.1.3.11 | 0.013 | - |
fructose 1,6-diphosphate | in presence of 0.01 mM phosphoenolpyruvate | Bacillus subtilis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | Mn2+ | irreversible inactivation in absence of Mn2+ | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 72000 | - |
x * 72000, SDS-PAGE | Bacillus subtilis |
| 3.1.3.11 | 380000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | Bacillus subtilis | constitutive enzyme | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.11 | - |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | constitutive enzyme | Bacillus subtilis | ? | - |
? | |
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | - |
Bacillus subtilis | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.11 | ? | x * 72000, SDS-PAGE | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 8 | - |
- |
Bacillus subtilis |
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