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Literature summary extracted from

  • Drake, J.C.; Baram, J.; Allegra, C.J.
    Isolation and characterization of a novel dihydrofolate formylating enzyme from human MCF-7 breast cancer cells (1990), Biochem. Pharmacol., 39, 615-618.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.17 Perchloric acid, 5%, denatures Homo sapiens
6.3.4.17 Perchloric acid, 5%, denatures Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.17 0.097
-
 dihydrofolate
-
 Homo sapiens
6.3.4.17 0.911
-
 dihydrofolate
-
 Lacticaseibacillus casei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.17 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.17 K+ enhances activity Homo sapiens
6.3.4.17 K+ enhances activity Lacticaseibacillus casei
6.3.4.17 Mg2+ required Homo sapiens
6.3.4.17 Mg2+ required Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.17 Homo sapiens
-
-
-
6.3.4.17 Lacticaseibacillus casei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.17 MCF-7 cell MCF-7 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.17 ATP + formate + dihydrofolate
-
 Homo sapiens ADP + phosphate + 10-formyldihydrofolate
-
 ?
6.3.4.17 ATP + formate + dihydrofolate
-
 Lacticaseibacillus casei ADP + phosphate + 10-formyldihydrofolate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.17 100
-
 60 s, denaturation Homo sapiens
6.3.4.17 100
-
 60 s, denaturation Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.17 8.5
-
-
 Homo sapiens
6.3.4.17 8.5
-
-
 Lacticaseibacillus casei