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Literature summary extracted from
- Purification and characterization of chitin deacetylase from Absidia coerulea (1995), J. Biochem., 117, 257-263.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.41 | synthesis | industrial production of chitosan | Absidia caerulea |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.41 | Fe3+ | - |
Absidia caerulea |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.41 | periplasmic space | - |
Absidia caerulea | 42597 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 75000 | - |
gel filtration, SDS-PAGE | Absidia caerulea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.41 | chitin + H2O | Absidia caerulea | - |
chitosan + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.41 | Absidia caerulea | - |
IFO 5301 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.41 | 516fold to homogeneity | Absidia caerulea |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.1.41 | chitin + H2O = chitosan + acetate | prefers nascent to preformed chitin | Absidia caerulea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.41 | mycelium | - |
Absidia caerulea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 11.37 | - |
- |
Absidia caerulea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.41 | -80°C | Absidia caerulea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.41 | chitin + H2O | - |
Absidia caerulea | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | colloidal chitin | Absidia caerulea | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Absidia caerulea | chitosan + acetate | - |
? | |
| 3.5.1.41 | N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Absidia caerulea | ? | - |
? | |
| 3.5.1.41 | N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Absidia caerulea | ? | - |
? | |
| 3.5.1.41 | N,N',N''-triacetylchitotriose + H2O | - |
Absidia caerulea | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.41 | monomer | 1 * 75000, SDS-PAGE | Absidia caerulea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 50 | - |
- |
Absidia caerulea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | additional information | - |
- |
Absidia caerulea |
| 3.5.1.41 | 50 | - |
stable for 1 h | Absidia caerulea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 5 | - |
- |
Absidia caerulea |
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